Rhodomyrtone decreases Staphylococcus aureus SigB activity during exponentially growing phase and inhibits haemolytic activity within membrane vesicles
| العنوان: | Rhodomyrtone decreases Staphylococcus aureus SigB activity during exponentially growing phase and inhibits haemolytic activity within membrane vesicles |
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| المؤلفون: | Watcharapong Mitsuwan, Irene Jiménez-Munguía, Monton Visutthi, Wipawadee Sianglum, Manuel J. Rodríguez-Ortega, Supayang P. Voravuthikunchai, A. Jover, F. Barcenilla, M. García, M. Pujol, O. Gasch, M.A. Domínguez, M. Camoez, C. Dueñas, E. Ojeda, J.A. Martínez, F. Marco, F. Chaves, M. Lagarde, F. López-Medrano, J.M. Montejo, E. Bereciertua, J.L. Hernández, M.Á. von Wichmann, A. Goenaga, J.M. García-Arenzana, B. Padilla, C. Padilla, E. Cercenado, G. García-Prado, J. Tapiol, J.P. Horcajada, M. Montero, M. Salvadó, A. Arnáiz, C. Fernández, E. Calbo, M. Xercavins, A. Granados, D. Fontanals, V. Pintado, E. Loza, J. Torre-Cisneros, R. Lara, F. Rodríguez-López, C. Natera, J.R. Blanco, I. Olarte, N. Benito, B. Mirelis, J. Murillas, E. Ruiz de Gopegui, H. Espejo, M.A. Morera, J. Rodríguez-Baño, L.E. López-Cortés, A. Pascual, C. Martín, J.A. Lepe, J. Molina, R. Sordé, B. Almirante, N. Larrosa |
| المساهمون: | Thailand Research Fund, Universidad de Córdoba (España) |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname MICROBIAL PATHOGENESIS r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
| بيانات النشر: | Elsevier BV, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Methicillin-Resistant Staphylococcus aureus, 0301 basic medicine, Staphylococcus aureus, SigB, Virulence Factors, Xanthones, 030106 microbiology, Virulence, Sigma Factor, Microbial Sensitivity Tests, Rhodomyrtone, medicine.disease_cause, Microbiology, 03 medical and health sciences, Minimum inhibitory concentration, Bacterial Proteins, Vancomycin, Sigma factor, medicine, Humans, Secretion, Minimum bactericidal concentration, biology, Chemistry, Cell Membrane, Staphylococcal Infections, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Membrane vesicles, Anti-Bacterial Agents, DNA-Binding Proteins, Haemolytic activity, 030104 developmental biology, Infectious Diseases, Mutation, bacteria, Bacteria, medicine.drug |
| الوصف: | REIPI/GEIH Study Group. Sigma factor B (SigB) controls the expression of Staphylococcus aureus genes including virulence factors and plays a role in the bacterial secretion system through membrane vesicle production. Inhibition of SigB could attenuate SigB dependent virulence and secretion system. The objective of this study was to determine the effects of rhodomyrtone on SigB and virulence factors related to SigB. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of rhodomyrtone against 67 clinical methicillin-resistant S. aureus isolates were 0.25–8 μg/ml, which were similar to those of vancomycin. Using luciferase gene fused to SigB dependent promoters of asp23, five time reduction in SigB activity was observed when the bacteria were treated with rhodomyrtone for 3 h. Rhodomyrtone significantly reduced SigB activity in a concentration dependent manner in exponentially growing cells (P This work was financially supported by The Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0033/2553) and TRF Senior Research Scholar (Grant No. RTA 6180006), the Thailand Research Fund. M.J.R.O. was funded by the 'XXII Programa Propio de Fomento de la Investigación', University of Córdoba. |
| تدمد: | 0882-4010 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bffd030238c921ec075fa351dcb3456Test https://doi.org/10.1016/j.micpath.2018.12.019Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1bffd030238c921ec075fa351dcb3456 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 08824010 |
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