Autophagic-lysosomal and mitochondrial sequestration of [14C]sucrose. Density gradient distribution of sequestered radioactivity
| العنوان: | Autophagic-lysosomal and mitochondrial sequestration of [14C]sucrose. Density gradient distribution of sequestered radioactivity |
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| المؤلفون: | Per Ottar Seglen, Helge Tolleshaug |
| المصدر: | European Journal of Biochemistry. 153:223-229 |
| بيانات النشر: | Wiley, 1985. |
| سنة النشر: | 1985 |
| مصطلحات موضوعية: | Male, Sucrose, Density gradient, Acid Phosphatase, Digitonin, Mitochondria, Liver, In Vitro Techniques, Mitochondrion, Biochemistry, chemistry.chemical_compound, Lysosome, Organelle, Autophagy, Centrifugation, Density Gradient, medicine, Animals, biology, Adenine, Metrizamide, Temperature, Acid phosphatase, Rats, Inbred Strains, Rats, Cytosol, medicine.anatomical_structure, chemistry, Isopycnic, Chromatography, Gel, biology.protein, Lysosomes, Subcellular Fractions |
| الوصف: | [14C]Sucrose, introduced into the cytosol of isolated rat hepatocytes by means of electropermeabilization, was sequestered by sedimentable subcellular particles during incubation of the cells at 37 degrees C. The sedimentation characteristics of particle-associated [14C]sucrose were different from the lysosomal marker enzyme acid phosphatase, suggesting an involvement of organelles of greater size than the average lysosome. Isopycnic banding in isotonic metrizamide/sucrose density gradients resolved two major peaks of radioactivity: a light peak (1.08-1.10 g/ml) coinciding with lysosomal marker enzymes, and a dense peak (1.15 g/ml), coinciding with a mitochondrial marker enzyme. The dense peak was preferentially associated with large-size particles having the sedimentation properties of mitochondria, and it was resistant to the detergent digitonin at a concentration which extracted all of the radioactivity in the light peak. Similarly the autophagy inhibitor 3-methyladenine prevented accumulation of [14C]sucrose in the light peak, while the radioactivity in the dense peak was unaffected. We therefore tentatively conclude that the light peak represents autophagic sequestration of [14C]sucrose into lysosomes (and probably autophagosomes) while the dense peak represents a mitochondrial uptake unrelated to autophagy. |
| تدمد: | 1432-1033 0014-2956 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc3d7372c99e63010b184e801dd8ae7cTest https://doi.org/10.1111/j.1432-1033.1985.tb09290.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....dc3d7372c99e63010b184e801dd8ae7c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321033 00142956 |
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