Furin Overexpression Suppresses Tumor Growth and Predicts a Better Postoperative Disease-Free Survival in Hepatocellular Carcinoma
| العنوان: | Furin Overexpression Suppresses Tumor Growth and Predicts a Better Postoperative Disease-Free Survival in Hepatocellular Carcinoma |
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| المؤلفون: | Chau-Ting Yeh, Chen-Hsin Liao, Kwang-Huei Lin, Yi-Hsin Tseng, Ya-Hui Huang, Ming-Wei Lai |
| المصدر: | PLoS ONE, Vol 7, Iss 7, p e40738 (2012) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Mouse, Angiogenesis, viruses, Gene Expression, lcsh:Medicine, Apoptosis, Amino Acid Chloromethyl Ketones, Metastasis, Mice, Molecular Cell Biology, lcsh:Science, Furin, Mice, Inbred BALB C, Multidisciplinary, biology, Liver Neoplasms, Animal Models, Middle Aged, Oncology, Hepatocellular carcinoma, embryonic structures, Medicine, Intercellular Signaling Peptides and Proteins, Female, Liver cancer, Research Article, medicine.medical_specialty, Carcinoma, Hepatocellular, animal structures, Cell Survival, Disease-Free Survival, Molecular Genetics, Model Organisms, Cell Line, Tumor, Internal medicine, Gastrointestinal Tumors, Genetics, medicine, Carcinoma, Animals, Humans, Gene Regulation, Biology, Cell Proliferation, Postoperative Care, Cell growth, business.industry, lcsh:R, Cancers and Neoplasms, Hepatocellular Carcinoma, medicine.disease, Xenograft Model Antitumor Assays, digestive system diseases, Endocrinology, Tumor progression, Cancer research, biology.protein, lcsh:Q, business |
| الوصف: | Furin is a member of the pro-protein convertase family. It processes several growth regulatory proteins into their active forms, which are critical to tumor progression, metastasis, and angiogenesis. Furin over-expression could occur in liver cancer and a previous study showed that over-expression of furin promoted HepG2 cell invasion in tail vein xenograft models. However, the clinical relevance of furin expression in hepatocellular carcinoma (HCC) remained unknown. Surprisingly, in a postoperative survival analysis for HCC patients, it was found that the tumor/non-tumor (T/N) ratio of furin expression ≥ 3.5 in HCC tissues predicted a better postoperative disease-free survival (DFS) (P = 0.010; log-rank test). Furthermore, subcutaneous xenograft experiments demonstrated a significant suppression effect of tumor growth in the furin-overexpressed xenografts (Huh7-Furin) compared to the mock control. Administration of a synthetic furin inhibitor for inhibition of the pro-protein convertase activity, decanoyl-Arg-Val-Lys-Arg-chloromethylketone (decRVKR-CMK), to the Huh7-Furin xenograft bearing mice restored the repression effect of tumor growth. In contrast, administration of decRVKR-CMK to the mock Huh7 xenograft bearing mice showed no change in growth rate. In conclusion, furin overexpression inhibited HCC tumor growth in a subcutaneous xenograft model and predicted a better postoperative DFS in clinical analysis. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac15ae3e0dd3cfd112a26ccff25d8ee7Test https://doi.org/10.1371/journal.pone.0040738Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ac15ae3e0dd3cfd112a26ccff25d8ee7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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