RdgBα reciprocally transfers PA and PI at ER–PM contact sites to maintain PI(4,5)P2 homoeostasis during phospholipase C signalling in Drosophila photoreceptors
| العنوان: | RdgBα reciprocally transfers PA and PI at ER–PM contact sites to maintain PI(4,5)P2 homoeostasis during phospholipase C signalling in Drosophila photoreceptors |
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| المؤلفون: | Shamshad Cockcroft, Evelyn Gomez-Espinoza, Shweta Yadav, Padinjat Raghu, Kathryn Garner |
| المصدر: | Biochemical Society Transactions. 44:286-292 |
| بيانات النشر: | Portland Press Ltd., 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Phosphatidylinositol 4,5-Diphosphate, 0301 basic medicine, Light Signal Transduction, Phospholipid, Phosphatidic Acids, Phospholipase, Biology, Endoplasmic Reticulum, Phosphatidylinositols, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, Pi, Animals, Drosophila Proteins, Homeostasis, Phosphatidylinositol, Caenorhabditis elegans, Eye Proteins, Phospholipase C, Endoplasmic reticulum, Cell Membrane, Membrane Proteins, Phosphatidic acid, Cell biology, Drosophila melanogaster, 030104 developmental biology, chemistry, Type C Phospholipases, Phosphorylation, Photoreceptor Cells, Invertebrate, Signal Transduction |
| الوصف: | Phosphatidylinositol (PI) is the precursor lipid for the synthesis of PI 4,5-bisphosphate [PI(4,5) P 2 ] at the plasma membrane (PM) and is sequentially phosphorylated by the lipid kinases, PI 4-kinase and phosphatidylinositol 4-phosphate (PI4 P )-5-kinase. Receptor-mediated hydrolysis of PI(4,5) P 2 takes place at the PM but PI resynthesis occurs at the endoplasmic reticulum (ER). Thus PI(4,5) P 2 resynthesis requires the reciprocal transport of two key intermediates, phosphatidic acid (PA) and PI between the ER and the PM. PI transfer proteins (PITPs), defined by the presence of the PITP domain, can facilitate lipid transfer between membranes; the PITP domain comprises a hydrophobic cavity with dual specificity but accommodates a single phospholipid molecule. The class II PITP, retinal degeneration type B (RdgB)α is a multi-domain protein and its PITP domain can bind and transfer PI and PA. In Drosophila photoreceptors, a well-defined G-protein-coupled phospholipase Cβ (PLCβ) signalling pathway, phototransduction defects resulting from loss of RdgBα can be rescued by expression of the PITP domain provided it is competent for both PI and PA transfer. We propose that RdgBα proteins maintain PI(4,5) P 2 homoeostasis after PLC activation by facilitating the reciprocal transport of PA and PI at ER–PM membrane contact sites. |
| تدمد: | 1470-8752 0300-5127 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1ef7d3ce483cbda98ec9951c97e9a3aTest https://doi.org/10.1042/bst20150228Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a1ef7d3ce483cbda98ec9951c97e9a3a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14708752 03005127 |
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