Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: Predictors of patient prognosis?
| العنوان: | Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: Predictors of patient prognosis? |
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| المؤلفون: | Fernando Vivanco, Aroa S. Maroto, C. Gómez-Alamillo, Angeles Ramos-Barron, Gloria Alvarez-Llamas, Laura Gonzalez-Calero, Alberto Ortiz, Manuel Arias, Marta Martin-Lorenzo, Jorge Ruiz-Criado |
| المساهمون: | UAM. Departamento de Medicina, Instituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS-FJD) |
| المصدر: | Biblos-e Archivo. Repositorio Institucional de la UAM instname Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Kininogen 1, Nephrology, Male, Pathology, 030232 urology & nephrology, Urine, Comorbidity, Gastroenterology, chemistry.chemical_compound, 0302 clinical medicine, Kininogen-1, Aged, 80 and over, Multidisciplinary, Molecules in urine, biology, Acute kidney injury, Acute Kidney Injury, Middle Aged, Prognosis, Creatinine, Female, Adult, medicine.medical_specialty, Adolescent, Medicina, Urinary system, Article, 03 medical and health sciences, Young Adult, Internal medicine, medicine, Humans, Aged, Retinol binding protein 4, business.industry, Kininogens, RBP4, medicine.disease, Retinol binding protein, 030104 developmental biology, chemistry, ROC Curve, biology.protein, Therapy, business, Urinary proteome, Retinol-Binding Proteins, Plasma, Biomarkers |
| الوصف: | Implementation of therapy for acute kidney injury (AKI) depends on successful prediction of individual patient prognosis. Clinical markers as serum creatinine (sCr) have limitations in sensitivity and early response. The aim of the study was to identify novel molecules in urine which show altered levels in response to AKI and investigate their value as predictors of recovery. Changes in the urinary proteome were here investigated in a cohort of 88 subjects (55 AKI patients and 33 healthy donors) grouped in discovery and validation independent cohorts. Patients'urine was collected at three time points: within the first 48 h after diagnosis(T1), at 7 days of follow-up(T2) and at discharge of Nephrology(T3). Differential gel electrophoresis was performed and data were confirmed by Western blot (WB), liquid chromatography/mass spectrometry (LC-MS/MS) and enzyme-linked immunosorbent assay (ELISA). Retinol binding protein 4 (RBP4) and kininogen-1 (KNG1) were found significantly altered following AKI. RBP4 increased at T1, and progressively decreased towards normalization. Maintained decrease was observed for KNG1 from T1. Individual patient response along time revealed RBP4 responds to recovery earlier than sCr. In conclusion, KNG1 and RBP4 respond to AKI. By monitoring RBP4, patient's recovery can be anticipated pointing to a role of RBP4 in prognosis evaluation. Funding: from Instituto de Salud Carlos III: FIS PI11/01401, PI13/01873, FIS IF08/3667-1, CP09/00229, PI13/00047, PI10/00624, ISCIII-RETIC REDinREN RD012/0021. FEDER funds, Comunidad de Madrid/CIFRA S2010/BMD-2378, Programa Intensificación Actividad Investigadora (ISCIII/Agencia Laín-Entralgo/CM) to AO, IDCSalud (3371/002) and Fundación Conchita Rábago de Jiménez Díaz, Proteomic Facility from Universidad Complutense de Madrid-Fundación Parque Científico de Madrid (UCM-FPCM), Spain, a member of ProteoRed-ISCIII Network member of ProteoRed- ISCIII Network |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d6cd3e634c2230b6122ebe1a742d888Test http://hdl.handle.net/10486/677997Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7d6cd3e634c2230b6122ebe1a742d888 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |