Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet
| العنوان: | Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet |
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| المؤلفون: | Silvia Martínez Llorens, Miguel Jover-Cerdá, Guillem Estruch, David S. Peñaranda, Gaspar Pérez Martínez, Ana Tomás-Vidal, Christine Bäuerl |
| المساهمون: | Ministerio de Economía y Competitividad (España), Generalitat Valenciana |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname International Journal of Molecular Sciences Volume 21 Issue 20 RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia International Journal of Molecular Sciences, Vol 21, Iss 7584, p 7584 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | gilthead seabream, 0301 basic medicine, 09.- Desarrollar infraestructuras resilientes, promover la industrialización inclusiva y sostenible, y fomentar la innovación, PRODUCCION ANIMAL, medicine.disease_cause, Catalysis, 14.- Conservar y utilizar de forma sostenible los océanos, mares y recursos marinos para lograr el desarrollo sostenible, Microbiology, lcsh:Chemistry, Inorganic Chemistry, Plant protein, 03 medical and health sciences, Immune system, Fish meal, plant protein, intestine explants culture, medicine, Physical and Theoretical Chemistry, Intestine explants culture, Gilthead seabream, lcsh:QH301-705.5, Molecular Biology, Spectroscopy, Vibrio alginolyticus, Inflammation, biology, Organic Chemistry, RT-qPCR, Pathogenic bacteria, 04 agricultural and veterinary sciences, General Medicine, biology.organism_classification, Computer Science Applications, 030104 developmental biology, Photobacterium damselae, lcsh:Biology (General), lcsh:QD1-999, inflammation, 040102 fisheries, ex vivo, 0401 agriculture, forestry, and fisheries, Ex vivo, Explant culture |
| الوصف: | The interaction between diet and intestinal health has been widely discussed, although in vivo approaches have reported limitations. The intestine explant culture system developed provides an advantage since it reduces the number of experimental fish and increases the time of incubation compared to similar methods, becoming a valuable tool in the study of the interactions between pathogenic bacteria, rearing conditions, or dietary components and fish gut immune response. The objective of this study was to determine the influence of the total substitution of fish meal by plants on the immune intestinal status of seabream using an ex vivo bacterial challenge. For this aim, two growth stages of fish were assayed (12 g): phase I (90 days), up to 68 g, and phase II (305 days), up to 250 g. Additionally, in phase II, the effects of long term and short term exposure (15 days) to a plant protein (PP) diet were determined. PP diet altered the mucosal immune homeostasis, the younger fish being more sensitive, and the intestine from fish fed short-term plant diets showed a higher immune response than with long-term feeding. Vibrio alginolyticus (V. alginolyticus) triggered the highest immune and inflammatory response, while COX-2 expression was significantly induced by Photobacterium damselae subsp. Piscicida (P. damselae subsp. Piscicida), showing a positive high correlation between the pro-inflammatory genes encoding interleukin 1β (IL1-β), interleukin 6 (IL-6) and cyclooxygenase 2(COX-2). The research was supported by a grant financed by the Spanish Ministerio de Economía y Competitividad AGL2015-70487-P and Generalitat Valenciana, IDIFEDER/2020/029 |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d76d64ac82ad6b90f18847be27a1a6aTest http://hdl.handle.net/10261/239154Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1d76d64ac82ad6b90f18847be27a1a6a |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |