Effect of Age and Lipoperoxidation in Rat and Human Adipose Tissue-Derived Stem Cells
| العنوان: | Effect of Age and Lipoperoxidation in Rat and Human Adipose Tissue-Derived Stem Cells |
|---|---|
| المؤلفون: | Francesco Marotta, Mercedes Cano, Mario Barbagallo, Antonio Ayala, Sandro Argüelles, Mario F. Muñoz |
| المساهمون: | Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular, Universidad de Sevilla. Departamento de Fisiología, Munoz M.F., Arguelles S., Marotta F., Barbagallo M., Cano M., Ayala A. |
| المصدر: | idUS: Depósito de Investigación de la Universidad de Sevilla Universidad de Sevilla (US) idUS. Depósito de Investigación de la Universidad de Sevilla instname Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2020 (2020) |
| بيانات النشر: | Hindawi, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Male, Homeobox protein NANOG, Aging, Time Factors, Stromal cell, Article Subject, Apoptosis, Biology, Regenerative Medicine, Biochemistry, Regenerative medicine, Cell therapy, AMP-Activated Protein Kinase Kinases, Peptide Elongation Factor 2, Sirtuin 1, SOX2, Animals, Humans, Rats, Wistar, Lipoperoxidation, Cell Proliferation, QH573-671, SOXB1 Transcription Factors, Stem Cells, Mesenchymal stem cell, Age Factors, Cell Differentiation, Mesenchymal Stem Cells, Nanog Homeobox Protein, Cell Biology, General Medicine, Middle Aged, Rats, Cell biology, Oxidative Stress, Adipose Tissue, age, Female, Lipid Peroxidation, Stem cell, Cytology, Protein Kinases, Research Article, HeLa Cells, Adult stem cell |
| الوصف: | A wide range of clinical applications in regenerative medicine were opened decades ago with the discovery of adult stem cells. Highly promising adult stem cells are mesenchymal stem/stromal cells derived from adipose tissue (ADSCs), primarily because of their abundance and accessibility. These cells have multipotent properties and have been used extensively to carry out autologous transplants. However, the biology of these cells is not entirely understood. Among other factors, the regeneration capacity of these cells will depend on both their capacity of proliferation/differentiation and the robustness of the biochemical pathways that allow them to survive under adverse conditions like those found in damaged tissues. The transcription factors, such as Nanog and Sox2, have been described as playing an important role in stem cell proliferation and differentiation. Also, the so-called longevity pathways, in which AMPK and SIRT1 proteins play a crucial role, are essential for cell homeostasis under stressful situations. These pathways act by inhibiting the translation through downregulation of elongation factor-2 (eEF2). In order to deepen knowledge of mesenchymal stem cell biology and which factors are determinant in the final therapeutic output, we evaluate in the present study the levels of all of these proteins in the ADSCs from humans and rats and how these levels are affected by aging and the oxidative environment. Due to the effect of aging and oxidative stress, our results suggest that before performing a cell therapy with ADSCs, several aspects reported in this study such as oxidative stress status and proliferation and differentiation capacity should be assessed on these cells. This would allow us to know the robustness of the transplanted cells and to predict the therapeutic result, especially in elder patients, where probably ADSCs do not carry out their biological functions in an optimal way. |
| وصف الملف: | application/pdf; text/xhtml |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ab80bc56689e56e4af1cf66231b8b66Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2ab80bc56689e56e4af1cf66231b8b66 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |