Genistein Inhibits Ca2+Influx by Extracellular ATP in PC12 Pheochromocytoma Cells
| العنوان: | Genistein Inhibits Ca2+Influx by Extracellular ATP in PC12 Pheochromocytoma Cells |
|---|---|
| المؤلفون: | Shinoda J, Atsushi Suzuki, Kozawa O |
| المصدر: | Hormone and Metabolic Research. 27:272-274 |
| بيانات النشر: | Georg Thieme Verlag KG, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Genistein, Protein tyrosine phosphatase, PC12 Cells, Biochemistry, Pheochromocytoma, chemistry.chemical_compound, Adenosine Triphosphate, Endocrinology, Internal medicine, medicine, Extracellular, Animals, Enzyme Inhibitors, Sodium orthovanadate, Protein kinase C, Chemistry, Calcium Radioisotopes, Biochemistry (medical), Ca2 influx, General Medicine, Protein-Tyrosine Kinases, medicine.disease, Isoflavones, Rats, Cell biology, Calcium, Protein Tyrosine Phosphatases, Vanadates, Tyrosine kinase |
| الوصف: | We previously showed that extracellular ATP-induced Ca2+ influx is inhibited by self-activated protein kinase C in rat PC12 pheochromocytoma cells. In the present study, we examined whether tyrosine kinase is involved in the ATP-induced Ca2+ influx in PC12 cells. Genistein, an inhibitor of protein tyrosine kinases, which by itself had little effect on 45Ca2+ influx, significantly suppressed the ATP-induced 45Ca2+ influx in a dose-dependent manner in the range between 1 and 30 micrograms/ml. Tyrophostin, another inhibitor of tyrosine kinases chemically distinct from genistein, also inhibited the 45Ca2+ influx. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, markedly enhanced the ATP-induced 45Ca2+ influx. These results suggest that tyrosine kinase regulates Ca2+ influx induced by extracellular ATP in PC12 pheochromocytoma cells. |
| تدمد: | 1439-4286 0018-5043 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dac49d22079f3d9d0b25892cf67f9257Test https://doi.org/10.1055/s-2007-979957Test |
| رقم الانضمام: | edsair.doi.dedup.....dac49d22079f3d9d0b25892cf67f9257 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14394286 00185043 |
|---|