Importance of miR-125a-5p and miR-122-5p expression in patients with HBV infection
| العنوان: | Importance of miR-125a-5p and miR-122-5p expression in patients with HBV infection |
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| المؤلفون: | Masood Garshasbi, Ramin Yaghobi, Ramin Lak |
| المصدر: | Cellular and Molecular Biology. 66:1-8 |
| بيانات النشر: | CMB Association, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Male, Hepatitis B virus, Small RNA, business.industry, Cell growth, Gene Expression Profiling, General Medicine, Middle Aged, medicine.disease_cause, Biomarker (cell), MicroRNAs, Hepatitis B, Chronic, Viral replication, microRNA, Immunology, medicine, MiR-122, Humans, Female, RNA extraction, business |
| الوصف: | MicroRNAs (miRNAs) as a small RNA and post-transcriptional modulator are shown to have regulatory effects for different cellular activities and pathways, such as metabolism, virus replication and also cell growth. In addition, miRNAs can regulate the replication of the hepatitis B virus (HBV). Therefore, the expression profile of miRNAs was evaluated in HBV infected patient groups and healthy controls. The expression levels of following microRNAs (as noninvasive biomarkers) were compared in two experimental (those with various stages of HBV infection) and control groups to evaluate their diagnosis ability: miR-122-5p, miR-125a-5p, miR-199a-3p, miR-210-5p, miR-205-5p, miR-155-5p, miR-372-5p, and miR-1-5p. RNA extraction was performed for 45 serum samples. The miRCURY LNAâ„¢ Universal RT-miRNA-PCR system and miRNA PCR panels were used for measuring microRNA expression profiles. To normalize quantitative values, the endogenous reference by UniSp6 expression was used.Serum miR-125a-5p and miR-122-5p were significantly higher in patients in different stages of HBV infection (p |
| تدمد: | 1165-158X 0145-5680 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b0a881a8b647ebc43e93295ab7e0625Test https://doi.org/10.14715/cmb/2020.66.5.1Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4b0a881a8b647ebc43e93295ab7e0625 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1165158X 01455680 |
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