Cellular changes that accompany shedding of human corneocytes
| العنوان: | Cellular changes that accompany shedding of human corneocytes |
|---|---|
| المؤلفون: | Melanie Hupe, Robert H. Rice, Debra Crumrine, Tzu-Kai Lin, Yoshikazu Uchida, Truus Roelandt, Peter M. Elias, Gemma Fabriàs, José Luis Abad, Juan-Luis Santiago, Larry Ackerman |
| المساهمون: | Surgical clinical sciences, Specialities, Skin function and permeability |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname The Journal of investigative dermatology, vol 132, iss 10 The Journal of investigative dermatology Lin, TK; Crumrine, D; Ackerman, LD; Santiago, JL; Roelandt, T; Uchida, Y; et al.(2012). Cellular changes that accompany shedding of human corneocytes. Journal of Investigative Dermatology, 132(10), 2430-2439. doi: 10.1038/jid.2012.173. UCSF: Retrieved from: http://www.escholarship.org/uc/item/95g757hfTest |
| بيانات النشر: | Blackwell Publishing, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Biochemistry, 030207 dermatology & venereal diseases, 0302 clinical medicine, cornified envelope, 0303 health sciences, Microscopy, abnormal desquamation, Dehydration, Chemistry, Bilayer, Desmoglein 1, Desmosomes, Exfoliation joint, Extracellular Matrix, Membrane, medicine.anatomical_structure, Intercellular Signaling Peptides and Proteins, Corneocyte desquamation, Adult, serine-protease activity, Clinical Sciences, Oncology and Carcinogenesis, Dermatology, desquamation, Electron, Article, 03 medical and health sciences, Fixatives, PERMEABILITY BARRIER HOMEOSTASIS, medicine, Extracellular, stratum corneum, Humans, skin barrier, Molecular Biology, ceramidase, 030304 developmental biology, Glycoproteins, Desmocollins, Corneocyte, electron microscopy, ruthenium tetroxide, corneodesmosomes, Dermatology & Venereal Diseases, human epidermal keratinocytes, desmocollin 1, Cell Biology, lamellar bilayers, cornified enveloppe, Microscopy, Electron, Ceramidase activity, Mammalian stratum corneum, Biophysics, Epidermis, X-linked ichthyosis, biochemical karakterization |
| الوصف: | Corneocyte desquamation has been ascribed to the following: 1) proteolytic degradation of corneodesmosomes (CDs); 2) disorganization of extracellular lamellar bilayers; and/or 3) swell-shrinkage-slough from hydration/dehydration. To address the cellular basis for normal exfoliation, we compared changes in lamellar bilayer architecture and CD structure in D-Squame strips from the first versus fifth stripping (outer vs. mid-stratum corneum (SC), respectively) from nine normal adult forearms. Strippings were either processed for standard electron microscopy (EM) or for ruthenium-, or osmium-tetroxide vapor fixation, followed by immediate epoxy embedment, an artifact-free protocol, which, to our knowledge, is previously unreported. CDs are largely intact in the mid-SC, but replaced by electron-dense (hydrophilic) clefts (lacunae) that expand laterally, splitting lamellar arrays in the outer SC. Some undegraded desmoglein 1/desmocollin 1 redistribute uniformly into corneocyte envelopes (CEs) in the outer SC (shown by proteomics, Z-stack confocal imaging, and immunoEM). CEs then thicken, likely facilitating exfoliation by increasing corneocyte rigidity. In vapor-fixed images, hydration only altered the volume of the extracellular compartment, expanding lacunae, further separating membrane arrays. During dehydration, air replaced water, maintaining the expanded extracellular compartment. Hydration also provoked degradation of membranes by activating contiguous acidic ceramidase activity. Together, these studies identify several parallel mechanisms that orchestrate exfoliation from the surface of normal human skin. © 2012 The Society for Investigative Dermatology. |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa6c412e44b73920d3adc5196a619aa4Test http://hdl.handle.net/10261/216659Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fa6c412e44b73920d3adc5196a619aa4 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |