Characterization of a Second Human Pulmonary Surfactant-associated Protein SP-A Gene
| العنوان: | Characterization of a Second Human Pulmonary Surfactant-associated Protein SP-A Gene |
|---|---|
| المؤلفون: | Gurmukh Singh, Sikandar L. Katyal, Joseph Locker |
| المصدر: | American Journal of Respiratory Cell and Molecular Biology. 6:446-452 |
| بيانات النشر: | American Thoracic Society, 1992. |
| سنة النشر: | 1992 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Pulmonary Surfactant-Associated Proteins, Proteolipids, Molecular Sequence Data, Restriction Mapping, Clinical Biochemistry, Biology, Exon, SFTPA2, Sequence Homology, Nucleic Acid, Complementary DNA, Humans, Coding region, Amino Acid Sequence, Lung, Molecular Biology, Gene, Glycoproteins, Genetics, Base Sequence, Pulmonary Surfactant-Associated Protein A, Nucleic acid sequence, Intron, Genetic Variation, Pulmonary Surfactants, Cell Biology, Blotting, Northern, Molecular biology, Genes, RNA, Tandem exon duplication, Oligonucleotide Probes |
| الوصف: | Pulmonary surfactant is a lipid-protein complex involved in maintaining alveolar stability. SP-A is the major surfactant-associated protein of 26 to 38 kD. A human SP-A gene (SP-A I) and two distinct SP-A cDNAs, MPSAP 1A and MPSAP 6A, have been reported previously. We have isolated and characterized a second human SP-A gene (SP-A II), which appears to code for the mRNA corresponding to the previously described MPSAP-1A cDNA. Both genes consist of five exons, a consensus recognition sequence for initiation, TATAAA, and a polyadenylation signal sequence. Significant divergence in the two genes is observed throughout. The divergence is highest in the upstream region, intron I, exon III, and noncoding portion of exon V. The coding regions of all other exons and the introns show much lower divergence. Transcripts from both genes were found in adult human lung, using gene-specific oligonucleotide probes in Northern blot analysis. |
| تدمد: | 1535-4989 1044-1549 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b169c62cc103db92cd806c9560cf6b4Test https://doi.org/10.1165/ajrcmb/6.4.446Test |
| رقم الانضمام: | edsair.doi.dedup.....0b169c62cc103db92cd806c9560cf6b4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15354989 10441549 |
|---|