Concentrations of Insulin Glargine and Its Metabolites During Long-Term Insulin Therapy in Type 2 Diabetic Patients and Comparison of Effects of Insulin Glargine, Its Metabolites, IGF-I, and Human Insulin on Insulin and IGF-I Receptor Signaling
| العنوان: | Concentrations of Insulin Glargine and Its Metabolites During Long-Term Insulin Therapy in Type 2 Diabetic Patients and Comparison of Effects of Insulin Glargine, Its Metabolites, IGF-I, and Human Insulin on Insulin and IGF-I Receptor Signaling |
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| المؤلفون: | Aimee J. Varewijck, Norbert Tennagels, Ronald Schmidt, Joseph A M J L Janssen, Hannele Yki-Järvinen |
| المساهمون: | Internal Medicine |
| المصدر: | Diabetes Diabetes, 62(7), 2539-2544. American Diabetes Association Inc. |
| بيانات النشر: | American Diabetes Association Inc., 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Insulin, Isophane, Insulin Glargine, 030209 endocrinology & metabolism, NPH insulin, Type 2 diabetes, Pathophysiology, Receptor, IGF Type 1, 03 medical and health sciences, 0302 clinical medicine, SDG 3 - Good Health and Well-being, Tandem Mass Spectrometry, Internal medicine, Internal Medicine, medicine, Humans, Hypoglycemic Agents, Insulin-Like Growth Factor I, Receptor, 030304 developmental biology, Original Research, 0303 health sciences, biology, Dose-Response Relationship, Drug, business.industry, Insulin glargine, Insulin, Autophosphorylation, nutritional and metabolic diseases, Middle Aged, medicine.disease, Receptor, Insulin, Insulin, Long-Acting, Insulin receptor, Endocrinology, Diabetes Mellitus, Type 2, biology.protein, lipids (amino acids, peptides, and proteins), Female, business, medicine.drug, Hormone, Chromatography, Liquid, Signal Transduction |
| الوصف: | We investigated 1) the ability of purified glargine (GLA), metabolites 1 (M1) and 2 (M2), IGF-I, and NPH insulin to activate the insulin receptor (IR)-A and IR-B and IGF-I receptor (IGF-IR) in vitro; 2) plasma concentrations of GLA, M1, and M2 during long-term insulin therapy in type 2 diabetic patients; and 3) IR-A and IR-B activation in vitro induced by serum from patients treated with GLA or NPH insulin. A total of 104 patients (age 56.3 ± 0.8 years, BMI 31.4 ± 0.5 kg/m2, and A1C 9.1 ± 0.1% [mean ± SE]) were randomized to GLA or NPH insulin therapy for 36 weeks. Plasma concentrations of GLA, M1, and M2 were determined by liquid chromatography–tandem mass spectrometry assay. IR-A, IR-B, and IGF-IR autophosphorylation was induced by purified hormones or serum by kinase receptor activation assays. In vitro, M1 induced comparable IR-A, IR-B, and IGF-IR autophosphorylation (activation) as NPH insulin. After 36 weeks, M1 increased from undetectable ( |
| وصف الملف: | application/pdf |
| تدمد: | 0012-1797 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d05a3b6f90fdb940f43b43910ecff38Test https://doi.org/10.2337/db12-1773Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5d05a3b6f90fdb940f43b43910ecff38 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00121797 |
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