Exosomal Proteins in the Aqueous Humor as Novel Biomarkers in Patients with Neovascular Age-related Macular Degeneration
| العنوان: | Exosomal Proteins in the Aqueous Humor as Novel Biomarkers in Patients with Neovascular Age-related Macular Degeneration |
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| المؤلفون: | Je Hyun Baek, Ae Jin Choi, Gum Yong Kang, Won-Chul Lee, Hyunjung Jade Lim, Soojin Yoon, Jeehyun Yoon, Joo Young Bang, Hye Won Chung, Hyung Soon Park, Soyoung Choi, Hyung Chan Kim |
| المصدر: | Journal of Proteome Research. 13:581-595 |
| بيانات النشر: | American Chemical Society (ACS), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Spectrometry, Mass, Electrospray Ionization, genetic structures, Cathepsin D, Biology, Exosomes, Biochemistry, Exosome, Cell Line, Aqueous Humor, Pathogenesis, Mice, medicine, Animals, Humans, Eye Proteins, Retina, Retinal pigment epithelium, General Chemistry, Macular degeneration, medicine.disease, eye diseases, Microvesicles, Mice, Inbred C57BL, medicine.anatomical_structure, Wet Macular Degeneration, Cancer research, Biomarker (medicine), sense organs, Biomarkers, Chromatography, Liquid |
| الوصف: | Age-related macular degeneration (AMD) describes the progressive degeneration of the retinal pigment epithelium (RPE), retina, and choriocapillaris and is the leading cause of blindness in people over 50. The molecular mechanisms underlying this multifactorial disease remain largely unknown. To uncover novel secretory biomarkers related to the pathogenesis of AMD, we adopted an integrated approach to compare the proteins identified in the conditioned medium (CM) of cultured RPE cells and the exosomes derived from CM and from the aqueous humor (AH) of AMD patients by LC-ESI-MS/MS. Finally, LC-MRM was performed on the AH from patients and controls, which revealed that cathepsin D, cytokeratin 8, and four other proteins increased in the AH of AMD patients. The present study has identified potential biomarkers and therapeutic targets for AMD treatment, such as proteins related to the autophagy-lysosomal pathway and epithelial-mesenchymal transition, and demonstrated a novel and effective approach to identifying AMD-associated proteins that might be secreted by RPE in vivo in the form of exosomes. The proteomics-based characterization of this multifactorial disease could help to match a particular marker to particular target-based therapy in AMD patients with various phenotypes. |
| تدمد: | 1535-3907 1535-3893 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5fa7e4715cbf78780af5432a5cfba5fTest https://doi.org/10.1021/pr400751kTest |
| رقم الانضمام: | edsair.doi.dedup.....e5fa7e4715cbf78780af5432a5cfba5f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15353907 15353893 |
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