دورية أكاديمية
ANALYSIS OF THE EXPRESSION LEVEL OF MICRORNA-29A IN PATIENTS WITH DIFFERENT CLINICAL VARIANTS OF CHRONIC HCV-INFECTION ; АНАЛІЗ РІВНЯ ЕКСПРЕСІЇ МІКРОРНК-29A У ХВОРИХ З РІЗНИМИ КЛІНІЧНИМИ ВАРІАНТАМИ ХРОНІЧНОЇ HCV-ІНФЕКЦІЇ
| العنوان: | ANALYSIS OF THE EXPRESSION LEVEL OF MICRORNA-29A IN PATIENTS WITH DIFFERENT CLINICAL VARIANTS OF CHRONIC HCV-INFECTION ; АНАЛІЗ РІВНЯ ЕКСПРЕСІЇ МІКРОРНК-29A У ХВОРИХ З РІЗНИМИ КЛІНІЧНИМИ ВАРІАНТАМИ ХРОНІЧНОЇ HCV-ІНФЕКЦІЇ |
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| المؤلفون: | Shevchenko-Makarenko , O. P. |
| المصدر: | Infectious Diseases – Infektsiyni khvoroby; No. 3 (2020); 29-35 ; Інфекційні хвороби; № 3 (2020); 29-35 ; 2414-9969 ; 1681-2727 ; 10.11603/1681-2727.2020.3 |
| بيانات النشر: | Ternopil National Medical University |
| سنة النشر: | 2020 |
| المجموعة: | Scientific Journals of Ternopil State Medical University / Наукові журнали Тернопільського державного медичного університету імені І.Я.Горбачевського |
| مصطلحات موضوعية: | chronic hepatitis C, HCV infection, liver fibrosis, liver cirrhosis, microRNA-29a, хронічний гепатит С, HCV-інфекція, фіброз печінки, цироз печінки, мікроРНК-29a |
| الوصف: | The purpose of the work was to analyze the baseline level of microRNA-29a expression in patients with chronic viral hepatitis C with genotype 1 of HCV, depending on the degree of liver fibrosis, the presence of cirrhosis, viral load of HCV, the duration of the disease, and other factors. Patients and methods: The study examined 74 patients with genotype 1 of chronic HCV infection with a mean age of (47.5±1.4) years. The control group consisted of 11 healthy individuals with negative markers for viral hepatitis, mean age (38.5±5.5) years. According to the degree of fibrosis on the METAVIR scale, patients were distributed as follows, number (%): F1 – 25 (33.8), F2 – 21 (28.4), F3 – 11 (14.9), F4 – 17 (22.9). Moreover, among patients with stage F4 fibrosis, only 10 (58.8%) patients had clinical signs of liver cirrhosis and stage A liver cirrhosis according to the Child-Pugh classification. The expression level of microRNA-29a (synonyms: miR-29a, hsa-miRNA-29a) was determined by a two-stage study according to the manufacturer’s protocol based on the Department of General and Molecular Pathophysiology of the Institute of Physiology, O.O. Bohomolets of the National Academy of Sciences of Ukraine, where after isolation of total RNA from blood plasma, reverse transcription was performed by quantitative real-time PCR using TaqMan® microRNA analysis (Applied Biosystems, USA). Statistical processing and analysis of data were performed using the software product Statistica v.6.1®. Results and conclusions. The results of the study showed that aberrant hyperexpression of microRNA-29a was detected in patients with chronic viral hepatitis C compared to healthy individuals: the median level of miR-29a expression in all patients was 20.6 times higher than in the group of healthy individuals and Log10 miR-29a in 4.9 times higher, respectively (p<0.001, U). We have not found significant difference expression level of microRNA-29a in patients according to gender (p=0.940), age (p=0.473), duration of the disease from the time of ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Ukrainian |
| العلاقة: | https://ojs.tdmu.edu.ua/index.php/inf-patol/article/view/11551/10969Test; https://ojs.tdmu.edu.ua/index.php/inf-patol/article/view/11551Test |
| DOI: | 10.11603/1681-2727.2020.3.11551 |
| الإتاحة: | https://doi.org/10.11603/1681-2727.2020.3.11551Test https://doi.org/10.11603/1681-2727.2020.3Test https://ojs.tdmu.edu.ua/index.php/inf-patol/article/view/11551Test |
| رقم الانضمام: | edsbas.3281934A |
| قاعدة البيانات: | BASE |
| DOI: | 10.11603/1681-2727.2020.3.11551 |
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