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1دورية أكاديمية
المؤلفون: Kolaković Ana, Bundalo Maja, Đurić Tamara, Končar Igor, Stanković Aleksandra, Živković Maja
المصدر: Vojnosanitetski Pregled, Vol 81, Iss 4, Pp 231-241 (2024)
مصطلحات موضوعية: carotid artery diseases, gene expression, plaque, atherosclerotic, proteins, renin angiotensin system, rna, messenger, Medicine (General), R5-920
الوصف: Background/Aim. The renin-angiotensin system (RAS) is linked to the development of atherosclerosis (As), including its initiation and progression. Besides the well-known angio-tensin-converting enzyme (ACE), two newer RAS family members are related to vascular remodeling – ACE2 as a homolog of ACE and collectrin [transmembrane protein 27 (TMEM27)] as a homolog of ACE2. Up to now, a limited number of studies have examined the expression of these RAS components in advanced carotid plaque (CP) tissue based on the sex of the patients and plaque phenotypes (PPs). There are two ultrasonographically defined PPs – the hypoechogenic plaque (HoP) and the hyperechogenic plaque (HerP) phenotype. The aim of the study was to investigate whether there was a correlation between the expression of RAS components in the CP and the sex and PPs of patients. Methods. We examined 74 patients with advanced CP who underwent carotid endarterectomy. The intraplaque expression of RAS components was determined with the real-time polymerase chain reaction, using the TaqMan® gene expression assays and Western blot. A two-way ANOVA followed by a post-hoc Tukey test was performed for the statistical analysis of results. Results. No interaction was recorded between the sex of the patients and PPs in influencing the relative expression of ACE and TMEM27 messenger RNA (mRNA) (p > 0.05). In 56.06% of plaque samples, no expression of ACE2 mRNA was detected. Among the plaques where ACE2 mRNA expression was detected, its expression level was higher in females with the HoP phenotype compared to females with the HerP phenotype (p < 0.001). In patients with the HoP phenotype, females had higher expression of ACE2 mRNA than males (p < 0.05). In the male study group, ACE protein levels were significantly lower in the HoP phenotype compared to the HerP phenotype (p < 0.001). Fe-males with the HoP phenotype had significantly higher ACE protein levels than males with the HoP phenotype (p < 0.0001). Conclusion. Our results revealed alterations in the expression levels of ACE and ACE2, at the mRNA and protein levels, in advanced carotid As. These alterations are impacted by sex and PP and may indicate a switch from the balanced RAS/ACE/ACE2 axis in the healthy blood vessel to the unbalanced axis in vascular remodeling due to As.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: de Araújo Melo Leonardo, Andrade de Santana Dandara, Schlaepfer Sales Caroline Brandi, Carneiro Braúlio Júnior, Martins Marília Trierveiler, Freitas Valéria Souza, Aquino Xavier Flávia Caló, Cury Patrícia Ramos, Gurgel Clarissa Araújo Silva, dos Santos Nunes Jean
المصدر: Srpski Arhiv za Celokupno Lekarstvo, Vol 151, Iss 1-2, Pp 15-20 (2023)
مصطلحات موضوعية: odontogenic cysts, ameloblastoma, stat3 transcription factor, gene expression, immunohistochemistry, Medicine
الوصف: Introduction/Objective. STAT3 (signal transducers and activators of transcription) is involved in different physiological processes, including cell proliferation and survival. High expression of this protein is observed in various types of cancer. This study aimed to investigate the gene and protein expression of STAT3 in a series of odontogenic cysts and tumors to provide more information about their biological profile. Methods. The STAT3 gene expression at mRNA was quantified by real-time quantitative polymerase chain reaction (RT-qPCR) in 23 odontogenic keratocysts (OKCs) and seven ameloblastomas (AMs), and compared to the non-neoplastic oral mucosa. We also assessed the expression of STAT3 gene at protein levels, using immunohistochemistry, in 43 OKCs and 47 AMs. Results. STAT3 transcripts were found in 96.6% of the tumors studied; however, the gene was downregulated in OKC and AM compared to the non-neoplastic oral mucosa. The STAT3 gene expression at mRNA level was higher in sporadic OKC than in syndromic OKC (p = 0.04). There was no difference in STAT3 gene expression at mRNA level between OKCs and AMs (p = 0.88). Immunostaining of STAT3 revealed no significant difference between sporadic and syndrome OKC (p > 0.05), nor between conventional and unicystic AMs (p > 0.05). Ameloblastomas exhibited significantly higher STAT3 immunostaining than OKCs (p = 0.03). In OKC and AM, STAT3 immunostaining was predominantly cytoplasmic and no difference in the cellular localization of STAT3 was observed between these lesions (p = 0.58). Conclusion. Our findings showed low expression of STAT3 gene in OKCs and AMs in relation to nonneoplastic oral mucosa. However, higher STAT3 immunostaining was observed in AMs compared to OKCs.
وصف الملف: electronic resource
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3كتاب
المؤلفون: Đurić, Marija
المساهمون: Pavlović, Sonja
المصدر: Trendovi u molekularnoj Biologiji
مصطلحات موضوعية: Impatiens walleriana, suša, akvaporini, genska ekspresija, drought, aquaporins, gene expression
الوصف: Povećanje prosečnih godišnjih temperatura iz godine u godinu za posledicu ostavlja sve izraženije sušne sezone. Suša nepovoljno utiče na rastenje i razviće biljaka, uzrokujući velike ekonomske gubitke na globalnom nivou. Efekti suše ispoljavaju se kako na morfološkom, tako i na fiziološkom, biohemijskom i molekularnom nivou organizacije biljaka. Izlaganje biljaka suši narušava normalan transport vode kroz ćelije što se odražava i na zastupljenost i funkciju kanala za vodu – akvaporina, na membranama. Akvaporini su transmembranski proteini koji formiraju kanale za prolazak vode i drugih molekula kroz ćelijske membrane. Ispoljavaju veoma značajnu ulogu u prilagođavanju protoka vode kroz ćelije shodno fiziološkom stanju. S obzirom na to, poslednjih godina velika pažnja posvećena je ulozi akvaporina pri odgovorima biljaka na dejstvo suše i drugih abiotičkih stresogenih faktora koji utiču na narušavanje vodnog režima biljaka. Istraživanja su usmerena ka praćenju promena u ekspresiji gena za akvaporine, zastupljenosti akvaporina na membranama i uticaju na usvajanje, transport i odavanje vode u atmosferu. U ovom radu predstavljene su analize strukture i regulacije ekspresije gena za akvaporine, kao i naučna istraživanja u proteklih deset godina o promenama u ekspresiji gena za akvaporine kod biljaka izlaganih suši. Poseban akcenat stavljen je na rezultate bioinformatičkih analiza akvaporina kod hortikulturne vrste Impatiens walleriana, i njihovu ekspresiju pri suši i rehidrataciji. ; The increase in average annual temperatures from year to year results in pronounced dry seasons. Drought adversely affects the plants growth and development, causing large economic losses on a global scale. The effects of drought are manifested both at the plant morphological, as well at the physiological, biochemical and molecular levels. Exposure of plants to drought disrupts the normal water transport through the cells, which is reflected in the presence and function of water channels - aquaporins, on the membranes. Aquaporins are ...
العلاقة: info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS//; https://imagine.imgge.bg.ac.rs/handle/123456789/2259Test; https://imagine.imgge.bg.ac.rs/bitstream/id/515844/TMB3_1-6,256-268,286-288.pdfTest; https://hdl.handle.net/21.15107/rcub_imagine_2259Test
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4دورية أكاديمية
المؤلفون: Stanišić-Zindović Jelena, Mihailović Branko, Đorđević Filip, Milovanović Marija, Arsenijević Nebojsa, Milovanović Jelena, Acović Aleksandar, Dubovina Dejan
المصدر: Vojnosanitetski Pregled, Vol 79, Iss 9, Pp 912-917 (2022)
مصطلحات موضوعية: bacterial outer membrane proteins, chaperonin 60, cytokines, gene expression, heat-shock proteins, interleukin 10, periapical diseases, tumor necrosis factor-alpha, Medicine (General), R5-920
الوصف: Background/Aim. Chronic microbial infections of the root canal are a common issue. This process very often causes an immune reaction in the root canal system that results in forming of chronic periapical lesions (PLs). The aim of this study was to determine the quantitative expression of the bacterial heat shock protein (HSP), chaperonin 60 (cpn60), and the pro-inflammatory and anti-inflammatory cytokines in periapical tissue obtained from individuals with chronic PLs and to determine if there is a correlation between the expression of the bacterial HSP and the expression of these cytokines. Methods. The study was performed on 18 PLs and 6 control samples of healthy periapical tissue, taken at the Department of Dentistry, Faculty of Medicine, University of Priština/Kosovska Mitrovica. The levels of messenger ribonucleic acid (mRNA) expression of pro- and anti-inflammatory cytokines and bacterial HSP were determined by quantitative real-time polymerase chain reaction (RT-PCR) and quantified by comparing to the internal control gene for glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Results. Analysis revealed significantly higher mRNA levels of tumor necrosis factor-alpha (TNF-a) and cpn60 in the tissue of PLs compared with normal periapical tissue (p < 0.05). Contrary to these results, the mRNA expression of anti-inflammatory interleukin-10 (IL-10) was significantly higher in the samples of normal periapical tissue compared with the mRNA levels of this cytokine in the tissue of PLs (p < 0.001). Expression of cpn60 is in strong correlation with TNF-a expression in PLs. Conclusion. cpn60 released from bacteria in periapical tissue could be a strong stimulator of inflammatory response and one of the important players in the pathogenesis of PLs.
وصف الملف: electronic resource
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5دورية أكاديمية
المصدر: Vojnosanitetski Pregled, Vol 79, Iss 10, Pp 1044-1047 (2022)
مصطلحات موضوعية: lymphoma, large b-cell, diffuse, drug therapy, gene expression, leukemia, hairy cell, hypercalcemia, immunotherapy, magnetic resonance imaging, mutation, tomography, emission-computed, Medicine (General), R5-920
الوصف: Introduction. Although secondary malignancies usually occur at different times after hairy cell leukemia (HCL) treatment, the occurrence of HCL and other malignancies at the same time is very rare. Synchronous HCL and diffuse large B-cell lymphoma (DLBCL) have not been described so far. Case report. The report presents a 62-year-old female patient with intense constitutional symptoms, hypercalcemia, pancytopenia, and osteolytic destruction of the left shoulder joint. Immunohistochemical analysis of the bone marrow revealed the presence of two cell populations: a population of HCL cells and a population of DLBCL cells with the expression of CMYC and BCL-2 proteins ("double expressor" DLBCL) and high proliferative activity (Ki-67+cells > 90%). Fluorescence in situ hybridization (FISH) analysis showed amplification of the BCL-2 gene. In addition, BRAF gene V600E mutation was detected. After intensive treatment with immunochemotherapy, radiotherapy, and bisphosphonates, the patient achieved complete remission, lasting for more than two years. Conclusion. As the association of HCL and lymphoma is very rare, diagnosis of synchronous occurrence of two lymphoproliferative diseases is a diagnostic and therapeutic challenge. It remains unclear whether DLBC and HCL originated from two different malignant clones or DLBCL developed by the transformation of HCL as the result of clonal evolution of the B-cell clone.
وصف الملف: electronic resource
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6مؤتمر
المؤلفون: Glavinić, Uroš, Jovanović, Nemanja M., Blagojević, Jovan, Ristanić, Marko, Stevanović, Jevrosima, Dominiković, Nina, Stanimirović, Zoran
المصدر: XXIV Simpozijum epizootiologa i epidemiologa /XXIV Epizootiološki dani, Subotica, 27 - 29. april, 2022
مصطلحات موضوعية: Apis mellifera, Nosema ceranae, тимол, оксидативни стрес, експресија имуно гена, thymol, oxidative stress, immune gene expression
الوصف: Микроспоридија Nosema ceranae је облигатни интрацелуларни паразит медоносне пчеле (Apis mellifera). Изазива болест ноземозу при чему оставља значајне последице на здравље, репродуктивне и продуктивне способности пчела. Тимол је састојак етеричног уља добијеног од тимијана (Thymus vulgaris) и многих других биљних врста. Користи се у еколошкој контроли пчелињег крпеља Varroa destructor, а у скорије време објављена су и истраживања потенцијалног ефекта тимола у сузбијању Nosema инфекције. У нашем истраживању, код пчела третираних тимолом и инфицираних ноземом праћен је број спора N. ceranae, нивои експресије гена значајних за имунитет пчела као и параметри оксидативног стреса. Тимол је апликован пре инфекције, у тренутку инфицирања, као и три дана након инфекције са N. ceranae. На крају експеримента, тимол конзумиран кроз шећерни сируп показао је антинозематозни ефекат доказан кроз број спора ноземе. Посматрањем нивоа експресије гена кроз време, примећује се да је у готово свим групама које су инфициране ноземом и третиране тимолом, за већину гена, ниво експресије растао. Супресија појединих гена (апидецина, абецин, дефензин) у групи која је примала тимол без инфекције ноземом, говори о потенцијалном имуносупресивном ефекту тимола код неинфицираних пчела. Активности антиоксидативних ензима и концентрације МДА биле су значајно ниже у групама које су током експеримента храњене уз додатак тимола, у односу на инфицирану групу. Наше истраживање доказало је позитивне ефекте тимола на здравље пчела инфицираних ноземом. Наиме, код пчела инфицираних ноземом, нису забележени негативни ефекти апликованог тимола, јер он у овом случају има терапијски (антинозематозни) ефекат, који је далеко значајнији од евентуалних пропратних нежељених дејстава. Међутим, утврдили смо да код пчела без инфекције ноземом, тимол може довести до појединих поремећаја, те треба бити обазрив са прекомерном и неконтролисаном употребом тимола у пчеларству. ; Nosema ceranae is an obligatory intracellular parasite of the honey bee (Apis mellifera). This ...
العلاقة: info:eu-repo/grantAgreement/MESTD/inst-2020/200143/RS//; https://vet-erinar.vet.bg.ac.rs/handle/123456789/3179Test; http://veterinar.vet.bg.ac.rs/bitstream/id/9405/bitstream_9405.pdfTest; https://hdl.handle.net/21.15107/rcub_veterinar_3179Test
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7مؤتمر
المؤلفون: Glavinić, Uroš, Ristanić, Marko, Jovanović, Nemanja M., Rajković, Milan, Niketić, Mia, Stevanović, Jevrosima, Stanimirović, Zoran
المساهمون: Gvozdić, Dragan
المصدر: Zbornik radova - XLIII seminar inovacija znanja veterinara
مصطلحات موضوعية: ekspresija gena, dijetetski suplementi, fumagilin, medonosna pčela, Nosema ceranae, oksidativni stres, gene expression, diet supplements, fumagillin, honey bee, oxidative stress
الوصف: donosne pčele (Apis mellifera). Izaziva bolest nozemozu (nozematozu) i u zavisnosti od stepena infekcije i jačine pčelinjih društava, ostavlja značajne posledice na zdravlje, reproduktivne i produktivne sposobnosti pčela. Antibiotik fumagilin je godinama bio jedini lek dostupan za kontrolu ovog patogena. Njegova primena dovedena je u pitanje zbog nedostataka koji prate njegovu primenu (efikasnost, mortalitet pčela i pojava rezidua u pčelinjim proizvodima). Širom sveta se sprovode istraživanja sa ciljem pronalaska alternative za kontrolu nozeme. U ovom radu prikazani su naši rezultati ispitivanja dijetetskih suplemenata na bazi prirodnih/biljnih ekstrakata (Medenko forte i ekstrakti gljiva Agaricus blazei i A. bisporus), timola i vitaminsko-mineralnog aditiva (BEEWELL AminoPlus). Kod pčela tretiranih svim nabrojanim suplementima i fumagilinom kao kontrolnim tretmanom, praćen je broj spora N. ceranae, nivoi ekspresije gena značajnih za imunitet pčela kao i parametri oksidativnog stresa. Svi preparati primenjivani su preventivno (tri dana pre infekcije), u trenutku inficiranja, kao i tri dana nakon infekcije sa N. ceranae, u cilju određivanja najboljeg momenta za primenu. Dokazani su negativni efekti fumagilina kada je preventivno primenjivan kod neinficiranih pčela. Kod inficiranih pčela većina testiranih suplemenata dovela je do smanjenja broja spora N. ceranae, a pokazali su i zaštitni efekat u smislu sprečavanja imunosupresije i oksidativnog stresa. ; The microsporidian Nosema ceranae is an obligate intestinal parasite of the honey bee (Apis mellifera). It causes nosemosis and, depending on the infection level and the strength of the infected bee colonies, causes significant health disorders, including malfunction of reproductive and productive capabilities of bees. The antibiotic fumagillin was the only drug available for Nosema control for many years. However, use of fumagillin has been questioned due to the drawbacks related to its efficacy, bee mortality and the presence of residues in bee products. Studies ...
العلاقة: info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//; Ugovor o realizaciji i finansiranju naučnoistraživačkog rada Fakulteta veterinarske medicine Univerziteta u Beogradu (Ugovor br: 451- 03-9/2021-14); https://vet-erinar.vet.bg.ac.rs/handle/123456789/2419Test; http://veterinar.vet.bg.ac.rs/bitstream/id/6703/bitstream_6703.pdfTest; https://hdl.handle.net/21.15107/rcub_veterinar_2419Test
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8رسالة جامعية
المؤلفون: Davidović Petar
المساهمون: Simeunović Jelica, Radnović Dragan, Đan Mihajla, Pavić Aleksandar, Blagojević Dajana
المصدر: CRIS UNS
مصطلحات موضوعية: Cijanobakterije, Artemia salina, Daphnia magna, Danio rerio, Ekspresija gena, Citotoksičnost, Genomika, Cyanobacteria, Gene expression, Cytotoxicity, Genomics
الوصف: Cijanobakterije predstavljaju raznovrsnu grupu mikroorganizama poznatih po produkciji visoko potentnih prirodnih toksina koji predstavljaju ozbiljnu pretnju po zdravlje ljudi, životinja i životne sredine. Ovi cijanotoksini se razlikuju po svojoj hemijskoj strukturi i mehanizmu delovanja, a često se u istom okruženju mogu naći različite klase, što čini procenu njihovih toksičnih efekata izazovnom. Kontinuiran razvoj i usavršavanje testova za precizniju detekciju, karakterizaciju i procenu rizika od cijanotoksina je glavni imperativ, te se na globalnom nivou sve češće naglašava potreba za pristupom koji bi omogućio sveobuhvatno proučavanje cijanotoksičnosti i praćenje efekata na različitim biološkim nivoima. U cilju rešavanja ovih izazova, razvijaju se različiti alternativni testovi i primenjuju model organizmi, među kojima su u fokusu u pogledu njihove korisnosti u unapređenju našeg razumevanja ove oblasti, vodeni kičmenjaci i beskičmenjaci. U skladu sa tim, formulisani su primarni ciljevi ove doktorske disertacije koji su podrazumevali procenu akutne toksičnosti ekstrakata odabranih sojeva cijanobakterija in vivo , kod akvatičnih beskičmenjaka ( Artemia salina i Daphnia magna ) i kičmenjaka ( Danio rerio ) i identifikovanje faktora koji dovode do modulacije njihove toksičnosti, uključujući dostupnost hranljivih materija, fotoperiod i faze rasta. Dodatno, istraženi su toksični efekti koji se manifestuju na molekularnom nivou dva model organizma, analizom relativnih promena u ekspresiji gena koji reaguju na toksine, kako bi se definisali neki od mehanizama toksičnog delovanja odabranih sojeva. Pored toga, in vitro test bazirani na upotrebi ćelijske linije humanog neuroblastoma je korišćen kako bi se odredio citotoksični i neurotoksični potencijal ispitanih sojeva. Konačno, jedan od osnovnih ciljeva istraživanja je podrazumevao ispitivanje metaboličkog potencijala odabranih sojeva da produkuju određene tipove cijanobakterijskih toksina koristeći imunotest, genomsku i bioinformatičku analizu. U seriji testova akutne ...
الإتاحة: https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija170489608879164.pdf?controlNumberTest=(BISIS)135726&fileName=170489608879164.pdf&id=22254&source=OpenAIRE&language=en
https://www.cris.uns.ac.rs/record.jsf?recordId=135726&source=OpenAIRE&language=enTest -
9رسالة جامعية
المؤلفون: Давидовић, Петар
المساهمون: Simeunović, Jelica, Radnović, Dragan, Đan, Mihajla, Pavić, Aleksandar, Blagojević, Dajana
المصدر: Универзитет у Новом Саду
مصطلحات موضوعية: Cijanobakterije, Artemia salina, Daphnia magna, Danio rerio, Ekspresija gena, Citotoksičnost, Genomika, Cyanobacteria, Gene expression, Cytotoxicity, Genomics
الوصف: Cijanobakterije predstavljaju raznovrsnu grupu mikroorganizama poznatih po produkciji visoko potentnih prirodnih toksina koji predstavljaju ozbiljnu pretnju po zdravlje ljudi, životinja i životne sredine. Ovi cijanotoksini se razlikuju po svojoj hemijskoj strukturi i mehanizmu delovanja, a često se u istom okruženju mogu naći različite klase, što čini procenu njihovih toksičnih efekata izazovnom. Kontinuiran razvoj i usavršavanje testova za precizniju detekciju, karakterizaciju i procenu rizika od cijanotoksina je glavni imperativ, te se na globalnom nivou sve češće naglašava potreba za pristupom koji bi omogućio sveobuhvatno proučavanje cijanotoksičnosti i praćenje efekata na različitim biološkim nivoima. U cilju rešavanja ovih izazova, razvijaju se različiti alternativni testovi i primenjuju model organizmi, među kojima su u fokusu u pogledu njihove korisnosti u unapređenju našeg razumevanja ove oblasti, vodeni kičmenjaci i beskičmenjaci. U skladu sa tim, formulisani su primarni ciljevi ove doktorske disertacije koji su podrazumevali procenu akutne toksičnosti ekstrakata odabranih sojeva cijanobakterija in vivo, kod akvatičnih beskičmenjaka (Artemia salina i Daphnia magna) i kičmenjaka (Danio rerio) i identifikovanje faktora koji dovode do modulacije njihove toksičnosti, uključujući dostupnost hranljivih materija, fotoperiod i faze rasta. Dodatno, istraženi su toksični efekti koji se manifestuju na molekularnom nivou dva model organizma, analizom relativnih promena u ekspresiji gena koji reaguju na toksine, kako bi se definisali neki od mehanizama toksičnog delovanja odabranih sojeva. Pored toga, in vitro test bazirani na upotrebi ćelijske linije humanog neuroblastoma je korišćen kako bi se odredio citotoksični i neurotoksični potencijal ispitanih sojeva. Konačno, jedan od osnovnih ciljeva istraživanja je podrazumevao ispitivanje metaboličkog potencijala odabranih sojeva da produkuju određene tipove cijanobakterijskih toksina koristeći imunotest, genomsku i bioinformatičku analizu. U seriji testova akutne ...
العلاقة: https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija170489608879164.pdf?controlNumberTest=(BISIS)135726&fileName=170489608879164.pdf&id=22254&source=NaRDuS&language=sr; https://www.cris.uns.ac.rs/record.jsf?recordId=135726&source=NaRDuS&language=srTest; https://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije170489609792283.pdf?controlNumberTest=(BISIS)135726&fileName=170489609792283.pdf&id=22255&source=NaRDuS&language=sr; https://nardus.mpn.gov.rs/handle/123456789/22339Test; http://nardus.mpn.gov.rs/bitstream/id/160275/Disertacija_15223.pdfTest; http://nardus.mpn.gov.rs/bitstream/id/160276/Izvestaj_komisije_15223.pdfTest; https://hdl.handle.net/21.15107/rcub_nardus_22339Test
الإتاحة: https://nardus.mpn.gov.rs/handle/123456789/22339Test
https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija170489608879164.pdf?controlNumberTest=(BISIS)135726&fileName=170489608879164.pdf&id=22254&source=NaRDuS&language=sr
https://www.cris.uns.ac.rs/record.jsf?recordId=135726&source=NaRDuS&language=srTest
https://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije170489609792283.pdf?controlNumberTest=(BISIS)135726&fileName=170489609792283.pdf&id=22255&source=NaRDuS&language=sr
http://nardus.mpn.gov.rs/bitstream/id/160275/Disertacija_15223.pdfTest
http://nardus.mpn.gov.rs/bitstream/id/160276/Izvestaj_komisije_15223.pdfTest
https://hdl.handle.net/21.15107/rcub_nardus_22339Test -
10دورية أكاديمية
المؤلفون: Stojković Ljiljana, Stanković Aleksandra, Životić Ivan, Dinčić Evica, Alavantić Dragan, Živković Maja
المصدر: Vojnosanitetski Pregled, Vol 77, Iss 9, Pp 967-973 (2020)
مصطلحات موضوعية: blood, chemokines, disease progression, gene expression, leukocytes, multiple sclerosis, rna, messenger, Medicine (General), R5-920
الوصف: Background/Aim. In vitro and in vivo studies show that CX3CL1 and CXCL16 chemokines and their specific receptors, CX3CR1 and CXCR6, respectively, mediate mechanism of neuroinflammation during the pathogenesis of multiple sclerosis (MS). The aim of this study was to investigate relative messenger ribonucleic acid (mRNA) levels of CX3CL1, CXCL16, CX3CR1 and CXCR6 in peripheral blood mononuclear cells, as potential molecular markers of relapsing-remitting (RR) MS. Methods. The study included 43 unrelated RR MS patients, 20 of them with clinically active disease (relapse) and 23 with clinically stable disease (remission), and 28 unrelated healthy subjects as controls. Real-time polymerase chain reactions (PCR) were performed using TaqMan® gene expression assays. Relative expression (mRNA) level of each target gene in each sample of peripheral blood mononuclear cells was calculated as the mean normalized expression. Results. The levels of CX3CR1 mRNA were significantly higher in clinically active RR MS patients compared to controls [fold change = 1.38, p (Mann-Whitney U test) = 0.009], and significantly lower in clinically stable vs active RR MS patients [fold change = - 1.43, p (t-test) = 0.03]. Stable RR MS patients had significantly higher CXCL16 mRNA levels than controls [fold change = 1.33, p (Mann-Whitney U test) = 0.006]. A trend of increased CXCR6 gene expression was found in active RR MS patients compared to controls [fold change = 1.23, p (Mann-Whitney U test) = 0.08]. In either active or stable RR MS patients there were no significant correlations of the clinical parameters with expression levels of the target genes. Conclusion. The current results show that increased CX3CR1 mRNA levels in peripheral blood mononuclear cells could represent a proinflammatory molecular marker of clinically active RR MS.
وصف الملف: electronic resource
