دورية أكاديمية
STAT3 gene expression in ameloblastomas and odontogenic keratocysts
| العنوان: | STAT3 gene expression in ameloblastomas and odontogenic keratocysts |
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| المؤلفون: | de Araújo Melo Leonardo, Andrade de Santana Dandara, Schlaepfer Sales Caroline Brandi, Carneiro Braúlio Júnior, Martins Marília Trierveiler, Freitas Valéria Souza, Aquino Xavier Flávia Caló, Cury Patrícia Ramos, Gurgel Clarissa Araújo Silva, dos Santos Nunes Jean |
| المصدر: | Srpski Arhiv za Celokupno Lekarstvo, Vol 151, Iss 1-2, Pp 15-20 (2023) |
| بيانات النشر: | Serbian Medical Society, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | odontogenic cysts, ameloblastoma, stat3 transcription factor, gene expression, immunohistochemistry, Medicine |
| الوصف: | Introduction/Objective. STAT3 (signal transducers and activators of transcription) is involved in different physiological processes, including cell proliferation and survival. High expression of this protein is observed in various types of cancer. This study aimed to investigate the gene and protein expression of STAT3 in a series of odontogenic cysts and tumors to provide more information about their biological profile. Methods. The STAT3 gene expression at mRNA was quantified by real-time quantitative polymerase chain reaction (RT-qPCR) in 23 odontogenic keratocysts (OKCs) and seven ameloblastomas (AMs), and compared to the non-neoplastic oral mucosa. We also assessed the expression of STAT3 gene at protein levels, using immunohistochemistry, in 43 OKCs and 47 AMs. Results. STAT3 transcripts were found in 96.6% of the tumors studied; however, the gene was downregulated in OKC and AM compared to the non-neoplastic oral mucosa. The STAT3 gene expression at mRNA level was higher in sporadic OKC than in syndromic OKC (p = 0.04). There was no difference in STAT3 gene expression at mRNA level between OKCs and AMs (p = 0.88). Immunostaining of STAT3 revealed no significant difference between sporadic and syndrome OKC (p > 0.05), nor between conventional and unicystic AMs (p > 0.05). Ameloblastomas exhibited significantly higher STAT3 immunostaining than OKCs (p = 0.03). In OKC and AM, STAT3 immunostaining was predominantly cytoplasmic and no difference in the cellular localization of STAT3 was observed between these lesions (p = 0.58). Conclusion. Our findings showed low expression of STAT3 gene in OKCs and AMs in relation to nonneoplastic oral mucosa. However, higher STAT3 immunostaining was observed in AMs compared to OKCs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Serbian |
| تدمد: | 0370-8179 2406-0895 |
| العلاقة: | https://doaj.org/toc/0370-8179Test; https://doaj.org/toc/2406-0895Test |
| DOI: | 10.2298/SARH220607115A |
| الوصول الحر: | https://doaj.org/article/47d962f0eb494733b8e92b6b467ab2edTest |
| رقم الانضمام: | edsdoj.47d962f0eb494733b8e92b6b467ab2ed |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 03708179 24060895 |
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| DOI: | 10.2298/SARH220607115A |