The aim of this study was to evaluate the level of knowledge about Ebola viral disease among medical students. A cross-sectional survey was conducted among fourth-year and sixth-year students at the faculties of medicine in Belgrade and Kragujevac. Anonymous questionnaire was used containing data about social and demographic characteristics of students and data about epidemiological and clinical characteristics of Ebola, possibility of its prevention and protection of health care workers. A total of 858 medical students (401 fourth-year and 457 sixth-year students) filled in the questionnaire. Fourth-year students showed better knowledge about fruit bats as a natural reservoir of Ebola virus (p