DICER and DROSHA gene expression in peripheral mononuclear blood cells from rheumatoid arthritis patients under methotrexate therapy
| العنوان: | DICER and DROSHA gene expression in peripheral mononuclear blood cells from rheumatoid arthritis patients under methotrexate therapy |
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| المؤلفون: | L. V. Topchieva, O. V. Balan, O. Y. Barysheva, I. M. Marusenko, I E Malysheva |
| المصدر: | Терапевтический архив, Vol 90, Iss 5, Pp 72-75 (2018) |
| بيانات النشر: | "Consilium Medicum" Publishing house, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, Ribonuclease III, rheumatoid arthritis, History, medicine.medical_specialty, drosha and dicer genes, microrna, Endocrinology, Diabetes and Metabolism, Gene Expression, lcsh:Medicine, Peripheral blood mononuclear cell, Gastroenterology, methotrexate, Arthritis, Rheumatoid, DEAD-box RNA Helicases, Internal medicine, microRNA, expression, medicine, Humans, RNA, Messenger, Gene, Drosha, Aged, biology, business.industry, lcsh:R, General Medicine, Middle Aged, medicine.disease, Peripheral, Rheumatoid arthritis, Leukocytes, Mononuclear, biology.protein, Methotrexate, Family Practice, business, medicine.drug, Dicer |
| الوصف: | The aim of this research was studying the level of DROSHA and DICER genes expression in peripheral mononuclear blood cells (PBMC) in rheumatoid arthritis (RA) patients under methotrexate therapy. Materials and methods. 82 people (from 45 to 70 years) enrolled in this study were divided into 3 groups (the first one - healthy donors (n=33 median age 49.93±1.87); the second group - rheumatoid arthritis patients without any therapy (n=15; median age 57.28±15.18) and the third group (n=34; median age 60.88±9.02) - rheumatoid arthritis patients who treated at least 4 weeks with methotrexate therapy (10-20 mg/week). The DICER and DROSHA genes expression level was determined by real-time PCR. Results. The number of DICER gene transcripts in PBMC in rheumatoid arthritis patients without therapy as in RA patients treated with methotrexate was reduced in comparison with the healthy donors (p 0.05 respectively). The level of DROSHA gene expression in PBMC was not significantly different in all groups enrolled in this study (p>0.05). Conclusion. Our findings suggest that that the DICER gene expression level in perifheral mononuclear blood cells decreased with the development of rheumatoid arthritis. Methotrexate doesn’t influence on the mRNA level of this gene. |
| اللغة: | Russian |
| تدمد: | 2309-5342 0040-3660 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0167c0e1503be3eb5041db324acb796fTest https://ter-arkhiv.ru/0040-3660/article/viewFile/32761/pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0167c0e1503be3eb5041db324acb796f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23095342 00403660 |
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