دورية أكاديمية
Evaluation of CAR-T cell cytotoxicity against a prostate carcinoma model
| العنوان: | Evaluation of CAR-T cell cytotoxicity against a prostate carcinoma model |
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| المؤلفون: | A.Kh. Valiullina, E.A. Zmievskaya, A.R. Rakhmatullina, M.N. Zhuravleva, E.E. Garanina, R.R. Miftakhova, A.V. Petukhov, A.A. Rizvanov, E.R. Bulatov |
| المصدر: | Učënye Zapiski Kazanskogo Universiteta. Seriâ Estestvennye Nauki, Vol 164, Iss 2, Pp 212-230 (2022) |
| بيانات النشر: | Kazan Federal University, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Science |
| مصطلحات موضوعية: | car-t cells, chimeric antigen receptor, solid tumor, cell model, prostate carcinoma, pc-3m, Science |
| الوصف: | In recent years, a new promising application of adoptive cell therapy (ACT) has been enabled by the development of technologies and the successful clinical use of T cells with a chimeric antigen receptor (CAR-T) for treating patients with malignant B-cell neoplasms. In this study, we obtained CAR-T cells with the second-generation chimeric antigen receptor FMC63-28Z-CAR. The effectiveness of the obtained CAR-T cells against the monolayer of the modified PC-3M prostate carcinoma cell line (Kat+CD19+) was evaluated. A correlation was demonstrated between an increased secretion of the proinflammatory cytokines IFNγ, TNFα, IL6 and a decreased confluence of the tumor cells monolayer. At the same time, a pronounced antitumor effect of CAR-T cells was observed starting from day 5 of coincubation. Thus, the proposed approach can potentially be applied for a preliminary evaluation of the effectiveness of biomedical CAR-T cell products in the treatment of solid tumors. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Russian |
| تدمد: | 2542-064X 2500-218X |
| العلاقة: | https://kpfu.ru/uz-eng-ns-2022-2-3.htmlTest; https://doaj.org/toc/2542-064XTest; https://doaj.org/toc/2500-218XTest |
| DOI: | 10.26907/2542-064X.2022.2.212-230 |
| الوصول الحر: | https://doaj.org/article/558856bff1e640c2879cb03e7df883a2Test |
| رقم الانضمام: | edsdoj.558856bff1e640c2879cb03e7df883a2 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 2542064X 2500218X |
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| DOI: | 10.26907/2542-064X.2022.2.212-230 |