دورية أكاديمية
Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography
| العنوان: | Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography |
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| المؤلفون: | Xikai Shu, Mei Wang, Daicheng Liu, Daijie Wang, Xiaojing Lin, Jianhua Liu, Xiao Wang, Luqi Huang |
| المصدر: | Química Nova, Vol 36, Iss 6, Pp 836-839 (2013) |
| بيانات النشر: | Sociedade Brasileira de Química, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Chemistry |
| مصطلحات موضوعية: | artichoke, HSCCC, polyphenols, Chemistry, QD1-999 |
| الوصف: | An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I), 24.5 mg of luteolin-7-O-β-D-rutinoside (compound II), 18.4 mg of luteolin-7-O-β-D-glucoside (compound III), and 33.4 mg of cynarin (compound IV) with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC) method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Spanish; Castilian Portuguese |
| تدمد: | 1678-7064 0100-4042 |
| العلاقة: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422013000600017&lng=en&tlng=enTest; https://doaj.org/toc/1678-7064Test |
| DOI: | 10.1590/S0100-40422013000600017 |
| الوصول الحر: | https://doaj.org/article/879b0bffc40d4363aef4786ebd700b87Test |
| رقم الانضمام: | edsdoj.879b0bffc40d4363aef4786ebd700b87 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16787064 01004042 |
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| DOI: | 10.1590/S0100-40422013000600017 |