دورية أكاديمية
The Effect of miR-372 on Genome Instability in MKN-45 Cell Line
| العنوان: | The Effect of miR-372 on Genome Instability in MKN-45 Cell Line |
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| المؤلفون: | Sorayya Ghasemi, Hossein Mozdarani, Masoud Soleimani |
| المصدر: | مجله دانشکده پزشکی اصفهان, Vol 32, Iss 311, Pp 2035-2047 (2015) |
| بيانات النشر: | Isfahan University of Medical Sciences, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Medicine LCC:Medicine (General) |
| مصطلحات موضوعية: | Gastric cancer (GC), miR-372, LATS2, Genomic instability, Medicine, Medicine (General), R5-920 |
| الوصف: | Background: Gastric cancer is one of the most common cancers in the world and the second leading cause of cancer mortality in humans. MicroRNAs are a group of endogenous RNA, small non-coding nucleotides in length of 21-23. Overexpression of miR-372 acts as an oncomir in various types of cancer via down-regulation of its target, LATS2. Down-regulation of LATS2 leads to the loss of cell cycle regulation, apoptosis inhibition, and increased proliferation rate of the cells. Methods: In this study, we increased the expression of miR-372 with lentivirus transduction inside the GC cell line MKN-45. After selection of positive cells, miR-372 and LATS2 expression levels were measured through real-time polymerase chain reaction (RT-PCR) assay. Cytochalasin B blocked (MN) assay was done to verify the presence or absence of MN for comparing genomic instability in treated cells compared to the controls. Findings: In the treated cells, compared with the controls, the amount of miR-372 expression significantly increased. Fold changes in 7, 14 and 21 days after the transduction were 7.85, 50.22 and 114.68, respectively (P = 0.030). In contrast to the control cells, the fold changes of LATS2 expression in these days were 0.39, 0.29 and 0.15, respectively (P = 0. 016). In addition, compared with control cells, the genomic instability of treated cells increased significantly (P < 0.001). Conclusion: These results indicate that in MKN-45 cell line, LATS2 is a target of miR-372. LATS2 is down-regulated with increased expression of miR-372. Reduce LATS2, leads to genomic instability during cell division and creates micronuclei and hence may be an important tumor suppressor. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Persian |
| تدمد: | 1027-7595 1735-854X |
| العلاقة: | http://jims.mui.ac.ir/index.php/jims/article/view/4636Test; https://doaj.org/toc/1027-7595Test; https://doaj.org/toc/1735-854XTest |
| الوصول الحر: | https://doaj.org/article/8d1537f61d574b34b4ce590b093283edTest |
| رقم الانضمام: | edsdoj.8d1537f61d574b34b4ce590b093283ed |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 10277595 1735854X |
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