العنوان البديل: Exercise-induced Oxidative Shift of Lactate Dehydrogenase Isoforms in Cerebrospinal Fluid of Male Wistar Rats. (English)

المؤلفون: حسینی, نسرین شیخ, نیکویی, روح الله, امینایی, محسن

المصدر: Arak Medical University Journal; 2018, Vol. 21 Issue 1, p52-62, 11p

مصطلحات موضوعية: CEREBROSPINAL fluid examination, PHYSICAL fitness, ANIMAL experimentation, ISOENZYMES, LACTATE dehydrogenase, RATS

مستخلص: Background: The aim of the present study was to investigate the effect of endurance training on the content of lactate dehydrogenase isoforms (LDHA and LDHB) and isozymes in cerebrospinal fluid of male wistar rats. Materials and Methods: Seventeen animals (5 weeks old) were randomly divided into two groups according their body weight: control (C; n = 9) and trained (T; n = 8). The animals from the T group were underwent a training program for 12 weeks (started at 20 m/min for 30 min and finished at 26 m/min for 60 min at the last week). Cerebrospinal fluid (CSF) samples were collected from cisterna Magna 72 hours after the last exercise session. LDH Isoforms and isozymes were measured by ELISA and Electrophoresis techniques, and the comparisons between groups were evaluated by student t-test. Results: Both LDHA and LDHB isoforms were found in the CSF of the C group; LDH1 and LDH5 had the highest and lowest content, respectively. Following the twelve-week training protocol, LDHB (p<0.01), LDH1 (p<0.01), and LDH2 concentrations (p<0.01) were significantly increased in the T group compared with those from the C group and LDH3 concentrations (p<0.01) and LDHA/LDHB ratio (p<0.01) were significantly decreased. Endurance training had no effect on LDHA and LDH4. Conclusion: Endurance training is associated with oxidative shift of lactate dehydrogenase isoforms and isozymes in cerebrospinal fluid which could be considered as a prelude to aerobic metabolism of lactate in the brain. [ABSTRACT FROM AUTHOR]

: Copyright of Arak Medical University Journal is the property of Arak University of Medical Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

العنوان البديل: The Role of Paraoxon Toxicity on Oxidative Stress Induction in Rat Heart and Spleen. (English)

المؤلفون: صالحي, مريم, جعفري, مهوش, عسگري, عليرضا, احمدي, صديق

المصدر: Journal of Zanjan University of Medical Sciences & Health Services; 2013, Vol. 21 Issue 84, p13-23, 11p

مصطلحات موضوعية: ANIMAL experimentation, BIOCHEMISTRY, CORN oil, DOSE-effect relationship in pharmacology, FREE radicals, GLUTATHIONE, HEART, INTRAPERITONEAL injections, LACTATE dehydrogenase, PHENOMENOLOGY, PARASYMPATHOMIMETIC agents, RATS, STATISTICAL sampling, SOLVENTS, SPLEEN, SUPEROXIDE dismutase, TRANSFERASES, OXIDATIVE stress, PHARMACODYNAMICS

مستخلص: Backgrounds and Objective: Paraoxon as the active form of parathion is one of the most of organophosphates (OPs) used in agriculture. Some OPs are capable of producing free radicals and inducing disturbance in the body antioxidant systems. The aim of this study was to investigate the effects of paraxaon on oxidative stress induction in the heart and spleen tissues in rats. Materials and Methods: Male Wistar rats were randomly divided into four groups (7 in each groups) as follows: control group received corn oil as paraoxon solvent, and the other three groups received different doses of paraxaon (0.3, 0.7, and 1mg/kg) by intraperitoneal injection. Animals were ether anesthesized 24 hours following the injection, their hearts and spleens were removed, and the levels of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and lactate dehydrogenase (LDH) activities, and glutathione (GSH) and malondialdehyde (MDA) were determined by biochemical methods. Results: Our data indicate that at doses higher than 0.3 mg/kg of paraoxon, SOD and GST levels significantly increases in both the heart and spleen. While there was also an increased CAT activity observed in the heart , both CAT and GSH levels showed a decrease in the spleen. Finally, a decreased LDH activity and increased MDA level in the heart tissue were observed at 1mg/kg dose of paraoxon. Conclusion: Paraoxon induces the production of free radicals and oxidative stress in a dose- dependent manner. The heart tissue appears to be more sensitive to the effects of paraoxon on oxidative stress induction compared to the spleen. [ABSTRACT FROM AUTHOR]

: Copyright of Journal of Zanjan University of Medical Sciences & Health Services is the property of Zanjan University of Medical Sciences & Health Services and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)