دورية أكاديمية
Frequent DNA hypomethylation of human juxtacentromeric BAGE loci in cancer.
| العنوان: | Frequent DNA hypomethylation of human juxtacentromeric BAGE loci in cancer. |
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| المؤلفون: | Grunau, Christoph, Sanchez, Cecilia, Ehrlich, Melanie, van der Bruggen, Pierre, Hindermann, Winfried, Rodriguez, Carmen, Krieger, Sophie, Dubeau, Louis, Fiala, Emerich, De Sario, Albertina |
| المساهمون: | UCL - SSS/DDUV - Institut de Duve |
| المصدر: | Genes, Chromosomes & Cancer, Vol. 43, no. 1, p. 11-24 (2005) |
| بيانات النشر: | JohnWiley & Sons, Inc. |
| سنة النشر: | 2005 |
| المجموعة: | DIAL@UCL (Université catholique de Louvain) |
| مصطلحات موضوعية: | Antigens, Neoplasm, Neoplasms, Ovarian Neoplasms, Polymerase Chain Reaction, Promoter Regions, Genetic, Restriction Mapping, Reverse Transcriptase Polymerase Chain Reaction, Spermatozoa, Testis, Breast Neoplasms, Cell Line, Tumor, DNA Methylation, DNA, Female, Gene Expression Regulation, Neoplastic, Humans, Male |
| الوصف: | The BAGE (B melanoma antigens) sequence family contains 15 nearly identical sequences that are in the juxtacentromeric regions of chromosomes 9, 13, 18, and 21. BAGE loci are expressed in male germ tissue and in a high percentage of cancers and cancer cell lines. We analyzed the DNA methylation state of the sequences in or near the promoters of the BAGE loci by a quantitative bisulfite and PCR-based assay (multiplex COBRA) using MboI and HphI in 18 somatic tissue samples, 4 testis and 4 sperm samples, and 48 tumors and tumor cell lines. In 94% of the control somatic tissue samples, DNA was highly methylated in the analyzed regions. In contrast, 98% of tumor DNA samples displayed hypomethylation. Also, DNA from testes and sperm was hypomethylated in at least one of the BAGE loci. BAGE transcripts were observed in only 47% of the analyzed tumor samples. Consequently, we propose BAGE hypomethylation as a new, highly informative epigenetic biomarker for the diagnosis of cancer, whose hypomethylation in cancer may be causally related to that of juxtacentromeric satellite DNA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | Ndonga |
| تدمد: | 1045-2257 1098-2264 |
| العلاقة: | boreal:141094; http://hdl.handle.net/2078.1/141094Test; info:pmid/15704127; urn:ISSN:1045-2257; urn:EISSN:1098-2264 |
| DOI: | 10.1002/gcc.20155 |
| الإتاحة: | https://doi.org/10.1002/gcc.20155Test http://hdl.handle.net/2078.1/141094Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.D6772858 |
| قاعدة البيانات: | BASE |
| تدمد: | 10452257 10982264 |
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| DOI: | 10.1002/gcc.20155 |