التفاصيل البيبلوغرافية
| العنوان: |
세포분열 과정에서의 Mis18α 인산화에 대한 기능 연구 ; Functional studies on the phosphorylation of Mis18α in the regulation of cell division |
| المؤلفون: |
이민경 |
| المساهمون: |
백성희, Minkyoung Lee, 자연과학대학 생명과학부 |
| بيانات النشر: |
서울대학교 대학원 |
| سنة النشر: |
2018 |
| المجموعة: |
Seoul National University: S-Space |
| مصطلحات موضوعية: |
Mis18α, Aurora B kinase, PLK1, Mitosis-specific phosphorylation, Polo Box Domain, kinetochore |
| الوقت: |
570 |
| الوصف: |
학위논문 (박사)-- 서울대학교 대학원 : 자연과학대학 생명과학부, 2018. 2. 백성희. ; Mis18α, a component of Mis18 complex comprising of Mis18α, Mis18β, and M18BP1, is known to localize at the centromere from late telophase to early G1 phase and plays a priming role in CENP-A deposition. Although its role in CENP-A deposition is well established, the other function of Mis18α remains unknown. Here, I elucidate a new function of Mis18α that is critical for the proper progression of cell cycle independent of its role in CENP-A deposition. I find that Aurora B kinase phosphorylates Mis18α during mitosis not affecting neither centromere localization of Mis18 complex nor centromere loading of CENP-A. However, the replacement of endogenous Mis18α by phosphorylation-defective mutant causes mitotic defects including micronuclei formation, chromosome misalignment, and the chromatin bridges or lagging chromatids. Interestingly, PLK1, another mitotic kinase, shows decreased recruitment in Mis18α phosphorylation-defective cell lines. PBD of PLK1 recognizes Mis18α phosphorylation so that its kinetochore recruitment can be enhanced. Together, my data demonstrate that Aurora B kinase-mediated mitotic phosphorylation of Mis18α is a crucial event for faithful cell cycle progression through the enhanced recruitment of PLK1 to the kinetochore. ; CHAPTER I. Introduction 1 I-1 Cell cycle 2 1.1 Introduction of cell cycle 2 1.2 Regulation of cell cycle 2 I-2 Aurora B kinase 6 2.1 Functions of Aurora B kinase in cell cycle 6 2.2 Substrates of Aurora B kinase 8 I-3 PLK1 10 3.1 Functions of PLK1 in cell cycle 10 3.2 Physical interaction with the substrates 13 I-4 Mis18α 16 4.1 Previous study on Mis18α 16 CHAPTER II. Mis18α is phosphorylated by Aurora B kinase during mitosis 19 II-1. Summary 20 II-2. Introduction 22 II-3. Results 24 II-4. Discussion 51 II-5. Materials and Methods 53 CHAPTER III. Phosphorylated Mis18αenhances PLK1 recruitment on kinetochore 58 III-1. Summary 59 III-2. Introduction 61 III-3. Results 62 III-4. Discussion 78 III-5. Mater Methods 81 CHAPTER ... |
| نوع الوثيقة: |
thesis |
| وصف الملف: |
application/pdf; 10200780 bytes |
| اللغة: |
Korean |
| العلاقة: |
000000149817; http://hdl.handle.net/10371/141135Test |
| الإتاحة: |
http://hdl.handle.net/10371/141135Test |
| رقم الانضمام: |
edsbas.5E30842B |
| قاعدة البيانات: |
BASE |
