التفاصيل البيبلوغرافية
| العنوان: |
A Highly Selective Mitochondria‐Targeting Fluorescent K + Sensor |
| المؤلفون: |
Kong, Xiangxing, Su, Fengyu, Zhang, Liqiang, Yaron, Jordan, Lee, Fred, Shi, Zhengwei, Tian, Yanqing, Meldrum, Deirdre R. |
| المساهمون: |
NIH |
| المصدر: |
Angewandte Chemie ; volume 127, issue 41, page 12221-12225 ; ISSN 0044-8249 1521-3757 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2015 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Regulation of intracellular potassium (K + ) concentration plays a key role in metabolic processes. So far, only a few intracellular K + sensors have been developed. The highly selective fluorescent K + sensor KS6 for monitoring K + ion dynamics in mitochondria was produced by coupling triphenylphosphonium, borondipyrromethene (BODIPY), and triazacryptand (TAC). KS6 shows a good response to K + in the range 30–500 m M , a large dynamic range ( F max / F 0 ≈130), high brightness ( ϕ f =14.4 % at 150 m M of K + ), and insensitivity to both pH in the range 5.5–9.0 and other metal ions under physiological conditions. Colocalization tests of KS6 with MitoTracker Green confirmed its predominant localization in the mitochondria of HeLa and U87MG cells. K + efflux/influx in the mitochondria was observed upon stimulation with ionophores, nigericin, or ionomycin. KS6 is thus a highly selective semiquantitative K + sensor suitable for the study of mitochondrial potassium flux in live cells. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
German |
| DOI: |
10.1002/ange.201506038 |
| الإتاحة: |
https://doi.org/10.1002/ange.201506038Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.A1F03A3D |
| قاعدة البيانات: |
BASE |
