The BTB-domain transcription factor ZBTB2 recruits chromatin remodelers and a histone chaperone during the exit from pluripotency
| العنوان: | The BTB-domain transcription factor ZBTB2 recruits chromatin remodelers and a histone chaperone during the exit from pluripotency |
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| المؤلفون: | Anaïs F. Bardet, Daniel Hess, Daniel Olivieri, Sébastien A. Smallwood, Ulrich Elling, Sujani Paramanathan, Joerg Betschinger |
| المساهمون: | Friedrich Miescher Institute for Biomedical Research (FMI), Novartis Research Foundation, Biotechnologie et signalisation cellulaire (BSC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche de l'Ecole de biotechnologie de Strasbourg (IREBS), Institute of Molecular Biotechnology (IMBA), Austrian Academy of Sciences (OeAW) |
| المصدر: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2021, 297 (2), pp.100947. ⟨10.1016/j.jbc.2021.100947⟩ The Journal of Biological Chemistry |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 2C, 2-cell embryo, TF, transcription factor, Aucun, BTB-link, extended BTB domain, Biochemistry, Ep400, chromatin remodeling, 0302 clinical medicine, i, inhibitor, Phylogeny, transcription factor, 2i, two inhibitors, Zinc finger, 0303 health sciences, LIF, leukemia inhibitory factor, bFGF, basic FGF, Zinc Fingers, KD, knock-down, Chromatin, Cell biology, N2B27, chemically defined base medium, Histone, BTB-POZ Domain, mESC, mouse embryonic stem cell, HiRA, histone regulator A, ChIP-seq, chromatin immunoprecipitation sequencing, EpiLC, epiblast-like cell, NuRD, nucleosome remodeling and deacetylase, Research Article, FDR, false discovery rate, Two-hybrid screening, DOX, doxycycline, Biology, Chromatin remodeling, 03 medical and health sciences, 2iLIF, N2B27 containing LIF, CHIR, and PD0325901, NuRD, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], MEK, mitogen-activated protein kinase kinase, Humans, CHIR, CHIR99021, Nucleosome, Histone Chaperones, Epigenetics, AP-MS, affinity purification–MS, Molecular Biology, Transcription factor, 030304 developmental biology, epigenetics, LFQ, label-free quantification, GSK3, glycogen synthetase kinase 3, PBST, PBS–0.1% Tween-20, Cell Biology, Y2H, yeast-2-hybrid, embryonic stem cell, HiRA, FGF, fibroblast growth factor, Repressor Proteins, ZBTB2, serum-LIF, fetal calf serum–containing medium supplemented with LIF, histone chaperone, BTB, broad-complex, tramtrack, and bric-a-brac, biology.protein, qPCR, quantitative PCR, Znf, zinc-finger domain, 030217 neurology & neurosurgery, Transcription Factors |
| الوصف: | Transcription factors (TFs) harboring broad-complex, tramtrack, and bric-a-brac (BTB) domains play important roles in development and disease. These BTB domains are thought to recruit transcriptional modulators to target DNA regions. However, a systematic molecular understanding of the mechanism of action of this TF family is lacking. Here, we identify the zinc finger BTB-TF Zbtb2 from a genetic screen for regulators of exit from pluripotency and demonstrate that its absence perturbs embryonic stem cell differentiation and the gene expression dynamics underlying peri-implantation development. We show that ZBTB2 binds the chromatin remodeler Ep400 to mediate downstream transcription. Independently, the BTB domain directly interacts with nucleosome remodeling and deacetylase and histone chaperone histone regulator A. Nucleosome remodeling and deacetylase recruitment is a common feature of BTB TFs, and based on phylogenetic analysis, we propose that this is a conserved evolutionary property. Binding to UBN2, in contrast, is specific to ZBTB2 and requires a C-terminal extension of the BTB domain. Taken together, this study identifies a BTB-domain TF that recruits chromatin modifiers and a histone chaperone during a developmental cell state transition and defines unique and shared molecular functions of the BTB-domain TF family. journal article research support, non-u.s. gov't 2021 08 2021 07 13 imported |
| اللغة: | French |
| تدمد: | 0021-9258 1083-351X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::026739c7c937a26878c6cad73fcd7ad9Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....026739c7c937a26878c6cad73fcd7ad9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219258 1083351X |
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