دورية أكاديمية
Copy number variation in the MSRB3 gene enlarges porcine ear size through a mechanism involving miR-584-5p
| العنوان: | Copy number variation in the MSRB3 gene enlarges porcine ear size through a mechanism involving miR-584-5p |
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| المؤلفون: | Congying Chen, Chenlong Liu, Xinwei Xiong, Shaoming Fang, Hui Yang, Zhiyan Zhang, Jun Ren, Yuanmei Guo, Lusheng Huang |
| المصدر: | Genetics Selection Evolution, Vol 50, Iss 1, Pp 1-18 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Animal culture LCC:Genetics |
| مصطلحات موضوعية: | Animal culture, SF1-1100, Genetics, QH426-470 |
| الوصف: | Abstract Background The size and type of ears are important conformation characteristics that distinguish pig breeds. A significant quantitative trait locus (QTL) for ear size has been identified on SSC5 (SSC for Sus scrofa chromosome) but the underlying causative gene and mutation remain unknown. Thus, our aim was to identify the gene responsible for enlarged ears in pig. Results First, we narrowed down the QTL region on SSC5 to a 137.85-kb interval that harbors only the methionine sulfoxide reductase B3 (MSRB3) gene. Then, we identified a 38.7-kb copy number variation (CNV) that affects the last two exons of MSRB3 and could be the candidate causative mutation for this QTL. This CNV showed complete concordance with genotype at the QTL of the founder animals in a white Duroc × Erhualian F2 intercross and was found only in pigs from six Chinese indigenous breeds with large ears and from the Landrace breed with half-floppy ears. Moreover, it accounted for the significant association with ear size on SSC5 across the five pig populations tested. eQTL mapping revealed that this CNV was significantly associated with the expression of the microRNA (miRNA) miR-584-5p, which interacts with MSRB3, one of its target genes. In vivo and in vitro experiments confirmed that miR-584-5p inhibits the translation of MSRB3 mRNA. Taken together, these results led us to conclude that presence of the 38.7-kb CNV in the genome of some pig breeds affects ear size by altering the expression of miR-584-5p, which consequently hinders the expression of one of its target genes (e.g. MSRB3). Conclusions Our findings shed insight into the underlying mechanism of development of external ears in mammals and contribute to a better understanding of how the presence of CNV can regulate gene expression. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | German English French |
| تدمد: | 1297-9686 |
| العلاقة: | http://link.springer.com/article/10.1186/s12711-018-0442-6Test; https://doaj.org/toc/1297-9686Test |
| DOI: | 10.1186/s12711-018-0442-6 |
| الوصول الحر: | https://doaj.org/article/f77e94eb1abd4e3fa7b7f7020f6986fdTest |
| رقم الانضمام: | edsdoj.f77e94eb1abd4e3fa7b7f7020f6986fd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12979686 |
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| DOI: | 10.1186/s12711-018-0442-6 |