التفاصيل البيبلوغرافية
| العنوان: |
Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters. |
| المؤلفون: |
McKenzie, Nancy L.1, Nodwell, Justin R.1 |
| المصدر: |
Journal of Bacteriology. Jul2007, Vol. 189 Issue 14, p16-16. 1p. |
| مصطلحات موضوعية: |
*CELLULAR signal transduction, *PHOSPHORYLATION, *ANTIBIOTICS, *STREPTOMYCES coelicolor, *PROMOTERS (Genetics), *CHROMATIN |
| مستخلص: |
The AbsA two-component signal transduction system, comprised of the sensor kinase AbsA1 and the response regulator AbsA2, acts as a negative regulator of antibiotic production in Streptomyces coelicolor, for which the phosphorylated form of AbsA2 (AbsA2∼P) is the agent of repression. In this study, we used chromatin immunoprecipitation to show that AbsA2 binds the promoter regions of actII-ORF4, cdaR, and redZ, which encode pathway-specific activators for actinorhodin, calcium-dependent antibiotic, and undecylprodigiosin, respectively. We confirm that these interactions also occur in vitro and that the binding of AbsA2 to each gene is enhanced by phosphorylation. Induced expression of actII-ORF4 and redZ in the hyperrepressive absA1 mutant (C542) brought about pathway-specific restoration of actinorhodin and undecylprodigiosin production, respectively. Our results suggest that AbsA2∼P interacts with as many as four sites in the region that includes the actII-ORF4 promoter. These data suggest that AbsA2∼P inhibits antibiotic production by directly interfering with the expression of pathway-specific regulators of antibiotic biosynthetic gene clusters. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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