المؤلفون: Gavars, Didzis, Gulbis, Egīls, Gavars, Mikus, Stašulāns, Jānis, Gavars, Valdis, Grundmane, Justīne, Stašulāne, Anna

المصدر: Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. ; volume 78, issue 1, page 99-105 ; ISSN 2255-890X

الوصف: In the current times of ever-growing prevalence of infectious diseases, it is requisite to explore ways to provide the safest and most effective medical care for our patients. The aim of this study is to explain how the issues raised by the SARS-CoV-2 pandemic were addressed by the E. Gulbis Laboratory in Latvia. The study looks back on the goal to introduce an automated and effective solution for the management of self-collected samples. The study is primarily aimed to formulate the conclusions about the data and use of automation in the self-sample kit collection. Results were collected from 18 automated (contactless) sample collection devices used by E. Gulbis Laboratory. Sixty-four thousand two hundred fifty-seven (64,257) saliva kits for SARSCoV-2 PCR testing were employed. It was found that 3.92% of them were positive (SARS-CoV-2 virus RNA found in saliva sample). The average processing time in automated devices located in the capital city was 11.13 hours, in the suburbs — 15.52 hours, rest of the country — 17.60 hours. The average age of patients that choose an automatic device to hand in their saliva sample kits was 33.94 years. These results suggest that by using the automated device, patient contacts are decreased, and direct communication with medical staff is excluded, which reduces the risk of infection during processing. Automated devices make sample kit distribution available 24 hours. They save workforce resources in the laboratory that are already very limited, especially during a pandemic period.

الإتاحة: https://doi.org/10.2478/prolas-2024-0014Test

المؤلفون: Gavars, Didzis, Perminov, Dmitry, Tauckels, Eriks, Lindenberga, Ilze, Miķelsone, Agnese, Auce, Agris

المصدر: Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. ; volume 78, issue 2, page 133-140 ; ISSN 2255-890X

الوصف: The reference intervals and median values of serum vitamin B12 level in blood from patients among the Latvian population were established for different age groups with a two-year step using data for a large number of patients, accumulated in E. Gulbis Laboratory in Latvia. The data represents the general population of Latvia. An indirect in silico method, developed in E. Gulbis laboratory for determination of serum vitamin B12 blood level reference intervals, was used. Strong elevation of serum vitamin B12 blood level was observed in children who were between 2 and 10 years old. The paper discusses the serum vitamin B12 values for children and a different serum vitamin B12 reference interval for young children is suggested.

الإتاحة: https://doi.org/10.2478/prolas-2024-0020Test

المؤلفون: Gavars, Didzis, Auce, Agris, Tauckels, Ēriks, Miķelsone, Agnese, Tutane, Anna Inese

المصدر: ENVIRONMENT. TECHNOLOGIES. RESOURCES. Proceedings of the International Scientific and Practical Conference; Vol 2 (2023): Environment. Technology. Resources. Proceedings of the 14th International Scientific and Practical Conference. Volume 2; 34-39 ; 2256-070X ; 1691-5402

مصطلحات موضوعية: B12 vitamin, myeloma, lymphocytic leukemia, myeloblastic leukemia, laboratory data

الوصف: Vitamin B12 blood level in patients with myeloma (C90 - International Classification of Diseases (ICD-10)), lymphocytic leukemia (C91) and myeloblastic leukemia (C92) prior and after the diagnosis and also BCR-ABL (fusion gene from breakpoint cluster region BCR gene and tyrosine-protein kinase ABL1 (Abelson murine leukemia) gene) tests for C92 patients were studied.Clinical records of 20 C92 patients in Riga East University Hospital were complemented with 6987 B12 clinical test data accumulated in E Gulbis laboratory (EGL) for 7451 patients over 20 years period. BCR-ABL and B12 dynamics for 11 patients with sufficient number of BCRABL and B12 tests were studied.Oracle Cloud with pseudonymized data replica from more than 350 000 000 original EGL clinical test data was used. The data were selected by online analytical processing and SQL built in tools and then used in offline analysis and visualization.Annually there are 107, 189 and 91 confirmed cases of C90, C91 and C92 in Latvia. EGL has 30% more C90-92 patients, due to suspected but later unconfirmed cases. Out of 7451 patients 1386 had one B12 test, two- 548, three and more- 864. The patients with diagnosis fluctuating between C90, C91 and C92 were excluded from the study. The data for the time period of 10 years before and after the first diagnosis were analyzed.Results. Methods and tools for data extraction and analysis from large amount of archived clinical test data were developed and applied. High and very high B12 level was observed for 53% of C92 patients starting from 3 years prior to diagnosis. For C90 and C91 patients B12 level changes around the diagnosis date were also observed although the effect was considerably smaller. Analysis of 11 selected patient data with clinical records showed timewise correlation between B12 and BCR-ABL for 3 of the patients.

وصف الملف: application/pdf

العلاقة: http://journals.ru.lv/index.php/ETR/article/view/7220/5948Test; http://journals.ru.lv/index.php/ETR/article/view/7220Test

الإتاحة: https://doi.org/10.17770/etr2023vol2.7220Test
http://journals.ru.lv/index.php/ETR/article/view/7220Test

المؤلفون: Sargautiene, Vanda, Leice, Alevtina, Gavars, Didzis

المصدر: Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. ; volume 76, issue 1, page 9-16 ; ISSN 2255-890X

الوصف: Iron deficiency (ID) and its late stage, iron deficiency anaemia, are often overlooked conditions in patients with inflammatory bowel disease (IBD). The study is primarily aimed at the need for first-phase screening for ID in patients with IBD using laboratory baseline parameters such as ferritin and C-reactive protein (CRP). Laboratory data of 4558 unique patients with IBD was analysed. For first-phase screening of ID, the thresholds of serum ferritin < 30 µg/l in the absence of inflammation (CRP < 5 mg/l) and serum ferritin < 100 µg/l in the presence of inflammation (CRP > 5 mg/l) were used. The study revealed that ferritin analysis was conducted for only one third of the enrolled unique patients with IBD over a five-year period. First-phase ID screening showed that decrease in ferritin values was found in 56% of unique IBD patients over this period, as determined using CRP and ferritin during the same blood collection (same referral number). Haemoglobin (Hb) tests were performed in 93% of unique IBD patients in the five-year period. A decrease in Hb was found in 21% of unique patients with Crohn’s disease, 20% of patients with ulcerative colitis, and in 5% of patients with unspecified non-infective gastroenteritis and colitis.

الإتاحة: https://doi.org/10.2478/prolas-2022-0002Test

المؤلفون: Gavars, Didzis, Perminov, Dmitry, Tauckels, Eriks, Lindenberga, Ilze, Tutāne, Anna, Auce, Agris

المصدر: Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. ; volume 76, issue 3, page 333-337 ; ISSN 2255-890X

الوصف: This study compared the reference intervals (RI) of B12 vitamin concentration in blood found in the literature with RIs extracted from data accumulated from a large number of patients by E. Gulbis Laboratory in Latvia. This paper investigated and demonstrated the possibility of using large amounts of random patient data to establish the RI for clinical laboratory tests. The blood level of B12 vitamin was selected as the model system for this study. The study used blind data for B12 blood level measurements from 132 379 patients accumulated in E. Gulbis Laboratory over a period of 15 years. In order to establish the reference intervals, the frequency distribution of log transformed B12 values was fit to a Gaussian distribution. The established B12 reference interval of 196 pg/ml and 942 pg/ml was found to be in good agreement with RIs reported elsewhere.

الإتاحة: https://doi.org/10.2478/prolas-2022-0050Test

المؤلفون: Konrāde, Ilze, Kalere, Ieva, Strēle, Ieva, Makrecka-Kūka, Marina, Veisa, Vija, Gavars, Didzis, Rezeberga, Dace, Pīrāgs, Valdis, Lejnieks, Aivars, Gruntmanis, Uģis, Neimane, Lolita, Liepiņš, Edgars, Dambrova, Maija

المصدر: Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. ; volume 71, issue 6, page 401-407 ; ISSN 1407-009X

الوصف: In the absence of a mandatory salt iodisation programme, two nationwide cross-sectional cluster surveys revealed persisting iodine deficiency among Latvian schoolchildren during the spring season and a noteworthy iodine deficiency in pregnant women in Latvia; these deficiencies warrant intervention. The consequences of mild-to-moderate iodine deficiency during pregnancy and lactation can adversely affect foetal brain development. Data from a Latvian population survey revealed the consumption of approximately 100 μg of iodine per day through foods and iodised salt. Therefore, strategies to increase the consumption of iodine-containing products should be implemented, particularly for children. In addition, to meet the increased iodine requirement during pregnancy, pregnant women should take daily supplements containing 150 μg iodine from the earliest time possible. All women of childbearing age should be advised to increase their dietary iodine intake by using iodised table salt and iodine-rich products: seafood, milk and milk products. For women with pre-existing thyroid pathologies, the medical decision should be considered on a case-by-case basis. Urinary iodine concentration monitoring among schoolchildren and pregnant women and neonatal thyrotropin registry analysis every five years would be an appropriate strategy for maintaining iodine intake within the interval that prevents iodine deficiency disorders.

الإتاحة: https://doi.org/10.1515/prolas-2017-0071Test
http://content.sciendo.com/view/journals/prolas/71/6/article-p401.xmlTest

المؤلفون: Sargautiene, Vanda, Gavars, Didzis, Ligere, Renate

المصدر: Clinical Diabetology; Vol 13, No 2 (2024); 106-115

مصطلحات موضوعية: type 2 diabetes (T2D), inflammatory bowel disease (IBD), comorbidities, C-reactive protein (CRP), ferritin, fecal calprotectin

الوصف: Objective: The current study aims to conduct comparative analysis of C-reactive protein (CRP), serum ferritin (SF), and fecal calprotectin (CALP) levels in individuals presenting with both inflammatory bowel disease (IBD) and type 2 diabetes (T2D) in contrast to those with IBD without T2D. Materials and methods: This retrospective analysis of a laboratory database included 2274 unique individuals diagnosed with IBD categorized into two cohorts: 2125 IBD patients without T2D, and 149 IBD patients with T2D. The differences between groups on a continuous measure were determined using non-parametric Mann-Whitney U test. Results: The study involved 925 male and 1200 female IBD patients without T2D, with mean ages of 41.6 ± 15.1 years for males and 47.1 ± 17.4 years for females. The second cohort involved 51 males and 98 females with IBD and T2D, with mean ages of 58.1 ± 13.9 years for males and 64.2 ± 12.1 years for females. Individuals with comorbid IBD and T2D demonstrated elevated levels of CRP and SF compared to those affected by IBD without T2D, with statistical significance observed (p < 0.05). An increase in CALP values was found in females afflicted with both IBD and T2D when compared to individuals with IBD without comorbid T2D (p < 0.01); however, such an increase was not noted in males. Conclusions: These findings underscore the need for further research on gender-specific differences and the potential presence of additional inflammatory conditions in individuals with IBD and T2D.

وصف الملف: application/pdf

العلاقة: https://journals.viamedica.pl/clinical_diabetology/article/view/99017Test

الإتاحة: https://doi.org/10.5603/cd.99017Test
https://journals.viamedica.pl/clinical_diabetology/article/view/99017Test

المؤلفون: Konrade, Ilze, Kalere, Ieva, Strele, Ieva, Makrecka-Kuka, Marina, Jekabsone, Anna, Tetere, Elina, Veisa, Vija, Gavars, Didzis, Rezeberga, Dace, Pīrāgs, Valdis, Lejnieks, Aivars, Dambrova, Maija

المصدر: Public Health Nutrition ; volume 18, issue 16, page 2990-2997 ; ISSN 1368-9800 1475-2727

الوصف: Objective Low iodine intake during pregnancy may cause thyroid dysfunction, which results in inadequate fetal brain development. In the absence of a universal salt iodization programme, we conducted a nationwide survey of iodine deficiency in pregnant women in Latvia. Design A countrywide twenty-cluster survey, with at least twenty women per cluster. Participants completed a questionnaire on dietary habits concerning iodine intake ( n 739). Thyroid function (thyroid-stimulating hormone, free thyroxine and thyroperoxidase antibodies) was measured ( n 550). Urinary iodine was measured using the ammonium persulfate method ( n 696). Setting The survey was performed in all regions of Latvia during the spring and autumn seasons in 2013. Subjects Pregnant women ( n 829). Results The median creatinine (Cr)-standardized urinary iodine concentration (UIC) was 80·8 (interquartile range (IQR) 46·1–130·6) µg/g Cr or 69·4 (IQR 53·9–92·6) µg/l during pregnancy, and 81 % of pregnant women had UIC levels below the WHO recommended range of 150–250 µg/g Cr. The UIC was lowest during the first trimester of pregnancy, 56·0 (IQR 36·4–100·6) µg/g Cr, reaching higher concentrations of 87·5 (IQR 46·4–141·7) µg/g Cr and 86·9 (IQR 53·8–140·6) µg/g Cr in the second and third trimesters, respectively. Women taking supplements containing ≥150 µg iodine (6·8 % of respondents) had non-significantly higher UIC than did women without supplementation (96·2 v. 80·3 µg/g Cr, respectively, P= NS). Thyroperoxidase antibody concentration did not correlate significantly with UIC: Spearman’s ρ =−0·012, P =0·78. Conclusions The median UIC indicates iodine deficiency in pregnant women in Latvia. Iodine supplementation (150 µg daily) and regular UIC monitoring should be suggested to overcome iodine deficiency and to reach the recommended levels without inducing autoimmune processes.

الإتاحة: https://doi.org/10.1017/s1368980015000464Test
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1368980015000464Test

المؤلفون: Leja, Marcis¹ cei@latnet.lv, Kojalo, Una¹, Frickauss, Gunars², Bandere, Biruta², Gavars, Didzis², Boka, Viesturs³

المصدر: Journal of Gastrointestinal & Liver Diseases. Jun2011, Vol. 20 Issue 2, p121-126. 6p. 2 Charts, 2 Graphs.

مصطلحات موضوعية: *SEROLOGY, *MEDICAL screening, *CELIAC disease in children, *ADULT-child relationships, *LABORATORY test panels, *DIAGNOSIS, *HEALTH

مصطلحات جغرافية: LATVIA

مستخلص: Background and Aims. A number of recent guidelines have discouraged the use of the old anti-gliadin tests for the detection of celiac disease; tissue transglutaminase IgA (tTGA) and anti-endomysial (EMA) tests are recommended instead. Our aim was to evaluate how the current recommendations have been applied in real practice. The secondary aim was to evaluate the positivity rates provided by different test types. Methods. We analyzed the number of celiac disease tests [anti-gliadin IgA (AGA), anti-gliadin IgG (AGG), tTGA and EMA] performed by the largest laboratory in Latvia. The analysis was performed on a yearly basis for the period between 2004 and 2009. Additionally, we analyzed the percentage of the positive test results for each of the tests. Results. The number of patients being tested for celiac disease constantly increased, with the average annual growth of 16.1%; this trend was similar both in children and in adults. The majority of patients (62.6%) were tested with anti-gliadin tests only; 27.7% were tested with either tTGA or EMA, while 9.7% were tested by a combination of the above groups. There was a substantial difference in the positivity rates of the different tests from 0.94% for EMA to 21.8% for AGG. Substantial differences were also present between various manufacturers' products. Conclusion. The current guidelines and the published evidence on the proper use of serological tests for celiac disease have been slow to be applied in clinical practice; more intensive education campaigns and change in reimbursement systems could improve the situation. Nevertheless, more clinicians in Latvia are checking patients for celiac disease; this suggests an overall increased awareness. [ABSTRACT FROM AUTHOR]