كتاب
أعرض في EDS

Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing

التفاصيل البيبلوغرافية
العنوان: Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing
المؤلفون: Mirbakhsh, M., Akhavan sepahy, A., Afsharnasab, M., Khanafari, A., Razavi, M.R.
سنة النشر: 2014
المجموعة: IODE-UNESCO: OceanDocs - E-Repository of Ocean Publications
مصطلحات موضوعية: Genetics, Vibriosis, Shrimp diseases, Litopenaeus vannamei, 16S rDNA sequencing, Molecular
جغرافية الموضوع: Iran
الوصف: A Gram-negative, rod-shaped, motile and halophilic bacterium identified as Vibrio harveyi was isolated from hatchery-reared larval of Iran Shrimp Research Centerfollowing mass mortality . Seven isolates of Vibrio spp.were collected from nauplii of Litopenaeus vannamei and then were evaluated for characteristics including morphology, physiology and biochemistry. The results indicated that the isolates belonged to a single species that grew on Thiosulfate citrate- Bile Sucrose agar and in 1.5 to 6% NaCl, positive lysine and ornithine decarboxylation and susceptible to O/129 disk. Identification of Vibrio harveyi strainIS01 (1 of 7 isolates) was confirmed by Polymerase Chain Reaction assay for Vibrio harveyi (expected amplicon 1493 bp). The 16S ribosomal DNA sequence (National Center for Biotechnology Information, GenBank accession number GU974342.1) gave 99% sequence identity to V. harveyi ATCC: 35084 (GenBank accession number EU130475.1). ; Published
نوع الوثيقة: book
article in journal/newspaper
وصف الملف: pp.384-393
اللغة: English
تدمد: 1562-2916
العلاقة: http://hdl.handle.net/1834/11727Test
الإتاحة: http://hdl.handle.net/1834/11727Test
رقم الانضمام: edsbas.D3573B95
قاعدة البيانات: BASE
الوصف
تدمد:15622916