المؤلفون: Mohammad Zarrabian, Jesús Montiel, Niels Sandal, Shaun Ferguson, Haojie Jin, Yen-Yu Lin, Verena Klingl, Macarena Marín, Euan K. James, Martin Parniske, Jens Stougaard, Stig U. Andersen

المصدر: Zarrabian, M, Montiel, J, Sandal, N, Ferguson, S, Jin, H, Lin, Y Y, Klingl, V, Marín, M, James, E K, Parniske, M, Stougaard, J & Andersen, S U 2022, ' A Promiscuity Locus Confers Lotus burttii Nodulation with Rhizobia from Five Different Genera ', Molecular plant-microbe interactions : MPMI, vol. 35, no. 11, pp. 1006-1017 . https://doi.org/10.1094/MPMI-06-22-0124-RTest

مصطلحات موضوعية: symbiotic nitrogen fixation, Physiology, Lotus, host range, General Medicine, genetic mapping, rhizobia, Agronomy and Crop Science

الوصف: Legumes acquire access to atmospheric nitrogen through nitrogen fixation by rhizobia in root nodules. Rhizobia are soil-dwelling bacteria and there is a tremendous diversity of rhizobial species in different habitats. From the legume perspective, host range is a compromise between the ability to colonize new habitats, in which the preferred symbiotic partner may be absent, and guarding against infection by suboptimal nitrogen fixers. Here, we investigate natural variation in rhizobial host range across Lotus species. We find that Lotus burttii is considerably more promiscuous than Lotus japonicus, represented by the Gifu accession, in its interactions with rhizobia. This promiscuity allows Lotus burttii to form nodules with Mesorhizobium, Rhizobium, Sinorhizobium, Bradyrhizobium, and Allorhizobium species that represent five distinct genera. Using recombinant inbred lines, we have mapped the Gifu/ burttii promiscuity quantitative trait loci (QTL) to the same genetic locus regardless of rhizobial genus, suggesting a general genetic mechanism for symbiont-range expansion. The Gifu/ burttii QTL now provides an opportunity for genetic and mechanistic understanding of promiscuous legume-rhizobia interactions. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::993e1d1e46ad5ba46e8dfda24e1cb2bdTest
https://pure.au.dk/portal/da/publications/a-promiscuity-locus-confers-lotus-burttii-nodulation-with-rhizobia-from-five-different-generaTest(e095499d-f426-4694-a3f7-65b45ee0b4b8).html

المؤلفون: Jane Thomas-Oates, Juan Liang, Yen-Yu Lin, Andreas Klingl, Emily Boul, Macarena Marín

المصدر: Journal of Experimental Botany

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Physiology, Lotus, ‘peg’-like structure, Context (language use), Plant Science, medicine.disease_cause, 01 natural sciences, Plant Root Nodulation, Rhizobium leguminosarum, Rhizobia, Microbiology, Nod factor, 03 medical and health sciences, medicine, nodulation, Symbiosis, intercellular infection, Rhizosphere, biology, Lotus burttii, root nodule symbiosis, fungi, food and beverages, biology.organism_classification, Research Papers, Mesorhizobium loti, Infection thread, 030104 developmental biology, Plant—Environment Interactions, Rhizobium, Root Nodules, Plant, 010606 plant biology & botany

الوصف: In the rhizosphere, legumes encounter mixed rhizobial communities. Lotus species exhibit dual infection mechanisms depending on the rhizobial strain that they encounter.
Lotus species develop infection threads to guide rhizobia into nodule cells. However, there is evidence that some species have a genetic repertoire to allow other modes of infection. By conducting confocal and electron microscopy, quantification of marker gene expression, and phenotypic analysis of transgenic roots infected with mutant rhizobia, we elucidated the infection mechanism used by Rhizobium leguminosarum Norway to colonize Lotus burttii. Rhizobium leguminosarum Norway induces a distinct host transcriptional response compared with Mesorhizobium loti. It infects L. burttii utilizing an epidermal and transcellular infection thread-independent mechanism at high frequency. The entry into plant cells occurs directly from the apoplast and is primarily mediated by ‘peg’-like structures, the formation of which is dependent on the production of Nod factor by the rhizobia. These results demonstrate that Lotus species can exhibit duality in their infection mechanisms depending on the rhizobial strain that they encounter. This is especially relevant in the context of interactions in the rhizosphere where legumes do not encounter single strains, but complex rhizobial communities. Additionally, our findings support a perception mechanism at the nodule cell entry interface, reinforcing the idea that there are successive checkpoints during rhizobial infection.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00f23b00f93952577eed943530d6cdbcTest
http://europepmc.org/articles/PMC6436148Test