Nap1 regulates proper CENP-B binding to nucleosomes
| العنوان: | Nap1 regulates proper CENP-B binding to nucleosomes |
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| المؤلفون: | Tachiwana, H., Miya, Y., Shono, N., Ohzeki, J., Osakabe, A., Otake, K., Larionov, V., Earnshaw, W. C., Kimura, Hiroshi, Masumoto, H., Kurumizaka, H. |
| المصدر: | Nucleic Acids Research Tachiwana, H, Miya, Y, Shono, N, Ohzeki, J-I, Osakabe, A, Otake, K, Larionov, V, Earnshaw, W C, Kimura, H, Masumoto, H & Kurumizaka, H 2013, ' Nap1 regulates proper CENP-B binding to nucleosomes ', Nucleic Acids Research, vol. 41, no. 5, pp. 2869-2880 . https://doi.org/10.1093/nar/gks1464Test |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Chromosomal Proteins, Non-Histone, DNA, Satellite/chemistry, Electrophoretic Mobility Shift Assay, Autoantigens, Histones/chemistry/metabolism, Histones, Nucleosomes/chemistry/*metabolism, 0302 clinical medicine, Histone methylation, Chromosomes, Human, Histone code, tRNA Methyltransferases, 0303 health sciences, biology, Nuclear Proteins, Nucleosomes, Cell biology, Neoplasm Proteins, Chromosomal Proteins, Non-Histone/chemistry/metabolism, Protein Transport, Histone, Chromosomes, Human/metabolism, Microtubule-Associated Proteins, Protein Binding, Satellite DNA, Centromere, Proteins/chemistry/*metabolism, macromolecular substances, DNA, Satellite, Gene Regulation, Chromatin and Epigenetics, Centromere Protein B/chemistry/*metabolism, 03 medical and health sciences, Histone H3, Centromere Protein A, Genetics, Nucleosome, Humans, 030304 developmental biology, Nuclear Proteins/metabolism, Autoantigens/chemistry/metabolism, Proteins, Molecular biology, Protein Structure, Tertiary, Chaperone (protein), biology.protein, Centromere/metabolism, Centromere Protein B, 030217 neurology & neurosurgery |
| الوصف: | CENP-B is a widely conserved centromeric satellite DNA-binding protein, which specifically binds to a 17-bp DNA sequence known as the CENP-B box. CENP-B functions positively in the de novo assembly of centromeric nucleosomes, containing the centromere-specific histone H3 variant, CENP-A. At the same time, CENP-B also prevents undesired assembly of the CENP-A nucleosome through heterochromatin formation on satellite DNA integrated into ectopic sites. Therefore, improper CENP-B binding to chromosomes could be harmful. However, no CENP-B eviction mechanism has yet been reported. In the present study, we found that human Nap1, an acidic histone chaperone, inhibited the non-specific binding of CENP-B to nucleosomes and apparently stimulated CENP-B binding to its cognate CENP-B box DNA in nucleosomes. In human cells, the CENP-B eviction activity of Nap1 was confirmed in model experiments, in which the CENP-B binding to a human artificial chromosome or an ectopic chromosome locus bearing CENP-B boxes was significantly decreased when Nap1 was tethered near the CENP-B box sequence. In contrast, another acidic histone chaperone, sNASP, did not promote CENP-B eviction in vitro and in vivo and did not stimulate specific CENP-B binding to CENP-A nucleosomes in vitro. We therefore propose a novel mechanism of CENP-B regulation by Nap1. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0305-1048 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f253be6930a0a2e85c9a521852385daTest http://t2r2.star.titech.ac.jp/cgi-bin/publicationinfo.cgi?q_publication_content_number=CTT100714620Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7f253be6930a0a2e85c9a521852385da |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03051048 |
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