Evidence for functional diversification within a fungal Nep1-like protein family
| العنوان: | Evidence for functional diversification within a fungal Nep1-like protein family |
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| المؤلفون: | Parthasarathy Santhanam, Bart P. H. J. Thomma, Isabell Albert, H. Peter van Esse, Thorsten Nürnberger, Luigi Faino |
| المصدر: | Molecular Plant-Microbe Interactions, 26(3), 278-286 Molecular Plant-Microbe Interactions 26 (2013) 3 |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Spores, Physiology, Arabidopsis, Nicotiana benthamiana, Verticillium, molecular characterization, Solanum lycopersicum, Arabidopsis thaliana, Phylogeny, Sequence Deletion, Genetics, Genome, biology, Virulence, EPS-2, plant-pathogen interactions, mycosphaerella-graminicola, food and beverages, General Medicine, Spores, Fungal, necrosis-inducing activity, Fungal, Multigene Family, fusarium-oxysporum, Genome, Fungal, carotovora subsp carotovora, Protein family, chitin-triggered immunity, Cell Survival, Hyphae, arabidopsis-thaliana, Host Specificity, Fungal Proteins, Botany, Tobacco, Gene family, verticillium-dahliae, Verticillium dahliae, Lycopersicon esculentum, Gene, Plant Diseases, Agronomy and Crop Science, fungi, biology.organism_classification, Laboratorium voor Phytopathologie, Laboratory of Phytopathology, npp1 domain |
| الوصف: | In this study, we functionally analyzed the gene family encoding necrosis- and ethylene-inducing-like proteins (NLP) of the vascular wilt pathogen Verticillium dahliae. We show that the composition of the NLP gene family varies little among V. dahliae isolates. The cytotoxic activity of NLP family members of a tomato-pathogenic V. dahliae strain was determined, demonstrating that only two of the seven NLP induced plant cell death. The genes encoding these cytotoxic NLP were found to be induced in V. dahliae upon colonization of tomato. Interestingly, targeted deletion of either of the two genes in V. dahliae significantly compromised virulence on tomato as well as on Arabidopsis plants, whereas deletion of only one of the two genes affected virulence on Nicotiana benthamiana. This could be attributed to differential induction of the two NLP genes in V. dahliae upon N. benthamiana colonization, revealing that the in planta induction of NLP genes varies between plant hosts. Intriguingly, one of the NLP genes appears to also affect vegetative growth and conidiospore production, because the corresponding deletion strain produced significantly fewer conidiospores and developed extensive aerial mycelium. In conclusion, we demonstrate that the expanded V. dahliae NLP family shows functional diversification, revealing not only differential cytotoxicity between family members but also that the cytotoxic NLP play a role in vegetative growth and asexual reproduction in addition to their contribution to virulence. |
| وصف الملف: | application/octet-stream; application/pdf |
| اللغة: | English |
| تدمد: | 0894-0282 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::deba63edfb89f5891703df764fab8bf6Test http://hdl.handle.net/11573/1056518Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....deba63edfb89f5891703df764fab8bf6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 08940282 |
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