التفاصيل البيبلوغرافية
| العنوان: |
In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR. |
| المؤلفون: |
Barreda-Manso, María Asunción, Nieto-Díaz, Manuel, Soto, Altea, Muñoz-Galdeano, Teresa, Reigada, David, Maza, Rodrigo M. |
| المصدر: |
International Journal of Molecular Sciences; Jun2021, Vol. 22 Issue 11, p6094, 1p |
| مصطلحات موضوعية: |
SARS-CoV-2, COVID-19 pandemic, MICRORNA, COVID-19, REPORTER genes, PLANT viruses |
| مستخلص: |
COVID-19 pandemic is caused by betacoronavirus SARS-CoV-2. The genome of this virus is composed of a single strand of RNA with 5′ and 3′-UTR flanking a region of protein-coding ORFs closely resembling cells' mRNAs. MicroRNAs are endogenous post-transcriptional regulators that target mRNA to modulate protein expression and mediate cellular functions, including antiviral defense. In the present study, we carried out a bioinformatics screening to search for endogenous human microRNAs targeting the 3′-UTR of SARS-CoV-2. Results from the computational techniques allowed us to identify 10 potential candidates. The capacity of 3 of them, together with hsa-miR-138-5p, to target the SARS-CoV-2 3′-UTR was validated in vitro by gene reporter assays. Available information indicates that two of these microRNAs, namely, hsa-miR-3941 and hsa-miR-138-5p, combine effective targeting of SARS-CoV-2 genome with complementary antiviral or protective effects in the host cells that make them potential candidates for therapeutic treatment of most, if not all, COVID-19 variants known to date. All information obtained while conducting the present analysis is available at Open Science Framework repository. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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