دورية أكاديمية
[6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice
| العنوان: | [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice |
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| المؤلفون: | Samad, Mehdi Bin, Mohsin, Md. Nurul Absar Bin, Razu, Bodiul Alam, Hossain, Mohammad Tashnim, Mahzabeen, Sinayat, Unnoor, Naziat, Muna, Ishrat Aklima, Akhter, Farjana, Kabir, Ashraf Ul, Hannan, J. M A |
| بيانات النشر: | BioMed Central |
| سنة النشر: | 2017 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | [6]-Gingerol, Leprdb/db mice, Type 2 diabetes, GLP-1, Rab27a, GLUT4, Glycogen synthase 1, Rab8, Rab10 |
| الوصف: | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0Test/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0Test/) applies to the data made available in this article, unless otherwise stated. ; Abstract Background [6]-Gingerol, a major component of Zingiber officinale, was previously reported to ameliorate hyperglycemia in type 2 diabetic mice. Endocrine signaling is involved in insulin secretion and is perturbed in db/db Type-2 diabetic mice. [6]-Gingerol was reported to restore the disrupted endocrine signaling in rodents. In this current study on Leprdb/db diabetic mice, we investigated the involvement of endocrine pathway in the insulin secretagogue activity of [6]-Gingerol and the mechanism(s) through which [6]-Gingerol ameliorates hyperglycemia. Methods Leprdb/db type 2 diabetic mice were orally administered a daily dose of [6]-Gingerol (200mg/kg) for 28days. We measured the plasma levels of different endocrine hormones in fasting and fed conditions. GLP-1 levels were modulated using pharmacological approaches, and cAMP/PKA pathway for insulin secretion was assessed by qRT-PCR and ELISA in isolated pancreatic islets. Total skeletal muscle and its membrane fractions were used to measure glycogen synthase 1 level and Glut4 expression and protein levels. Results 4-weeks treatment of [6]-Gingerol dramatically increased glucose-stimulated insulin secretion and improved glucose tolerance. Plasma GLP-1 was found to be significantly elevated in the treated mice. Pharmacological intervention of GLP-1 levels regulated the effect of [6]-Gingerol on insulin secretion. Mechanistically, [6]-Gingerol treatment upregulated and activated cAMP, ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1472-6882 |
| العلاقة: | BMC Complementary and Alternative Medicine, 17(1):395; https://hdl.handle.net/10371/137261Test |
| DOI: | 10.1186/s12906-017-1903-0 |
| الإتاحة: | https://doi.org/10.1186/s12906-017-1903-0Test https://hdl.handle.net/10371/137261Test |
| حقوق: | The Author(s). |
| رقم الانضمام: | edsbas.B12789E2 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14726882 |
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| DOI: | 10.1186/s12906-017-1903-0 |