Polarization of diploid daughter cells directed by spatial cues and GTP hydrolysis of Cdc42 budding yeast
| العنوان: | Polarization of diploid daughter cells directed by spatial cues and GTP hydrolysis of Cdc42 budding yeast |
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| المؤلفون: | Mid Eum Lee, Wing-Cheong Lo, Ching-Shan Chou, Hay-Oak Park, Monisha Narayan |
| المصدر: | PLoS ONE, Vol 8, Iss 2, p e56665 (2013) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cell division, Saccharomyces cerevisiae, Signaling in cellular processes, lcsh:Medicine, Yeast and Fungal Models, CDC42, Signal transduction, Cell Growth, 03 medical and health sciences, 0302 clinical medicine, Model Organisms, Molecular cell biology, Cell polarity, Genetics, lcsh:Science, Biology, GTPase signaling, 030304 developmental biology, Cytokinesis, cdc42 GTP-Binding Protein, Saccharomyces cerevisiae, 0303 health sciences, Budding, Multidisciplinary, biology, Hydrolysis, Systems Biology, lcsh:R, Cell Polarity, Computational Biology, Models, Theoretical, biology.organism_classification, Diploidy, Cell biology, Cdc42 GTP-Binding Protein, Cytoplasm, lcsh:Q, Guanosine Triphosphate, Gene Function, 030217 neurology & neurosurgery, Cell Division, Research Article |
| الوصف: | Cell polarization occurs along a single axis that is generally determined by a spatial cue. Cells of the budding yeast exhibit a characteristic pattern of budding, which depends on cell-type-specific cortical markers, reflecting a genetic programming for the site of cell polarization. The Cdc42 GTPase plays a key role in cell polarization in various cell types. Although previous studies in budding yeast suggested positive feedback loops whereby Cdc42 becomes polarized, these mechanisms do not include spatial cues, neglecting the normal patterns of budding. Here we combine live-cell imaging and mathematical modeling to understand how diploid daughter cells establish polarity preferentially at the pole distal to the previous division site. Live-cell imaging shows that daughter cells of diploids exhibit dynamic polarization of Cdc42-GTP, which localizes to the bud tip until the M phase, to the division site at cytokinesis, and then to the distal pole in the next G1 phase. The strong bias toward distal budding of daughter cells requires the distal-pole tag Bud8 and Rga1, a GTPase activating protein for Cdc42, which inhibits budding at the cytokinesis site. Unexpectedly, we also find that over 50% of daughter cells lacking Rga1 exhibit persistent Cdc42-GTP polarization at the bud tip and the distal pole, revealing an additional role of Rga1 in spatiotemporal regulation of Cdc42 and thus in the pattern of polarized growth. Mathematical modeling indeed reveals robust Cdc42-GTP clustering at the distal pole in diploid daughter cells despite random perturbation of the landmark cues. Moreover, modeling predicts different dynamics of Cdc42-GTP polarization when the landmark level and the initial level of Cdc42-GTP at the division site are perturbed by noise added in the model. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::790a3f8129a1a0d6bca860389542bc1aTest http://europepmc.org/articles/PMC3577668?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....790a3f8129a1a0d6bca860389542bc1a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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