دورية أكاديمية
Requirement for Msh6, but not for Swi4 (Msh3), in Msh2-dependent repair of base-base mismatches and mononucleotide loops in Schizosaccharomyces pombe
| العنوان: | Requirement for Msh6, but not for Swi4 (Msh3), in Msh2-dependent repair of base-base mismatches and mononucleotide loops in Schizosaccharomyces pombe |
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| المؤلفون: | Tornier, C., Bessone, S., Varlet, I., Rudolph, C., Darmon, M., Fleck, O. |
| المساهمون: | Laboratory of Medical Biochemistry, University of Bordeaux 2, University of Bordeaux Segalen , Bordeaux, France |
| المصدر: | ISSN: 0016-6731 ; Genetics ; https://hal.science/hal-02491014Test ; Genetics, 2001, 158 (1), pp.65-75. |
| بيانات النشر: | HAL CCSD Oxford University Press |
| سنة النشر: | 2001 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics |
| الوصف: | International audience ; The msh6 mismatch repair gene of Schizosaccharomyces pombe was cloned, sequenced, and inactivated. Strains bearing all combinations of inactivated msh6, msh2, and swi4 (the S. pombe MSH3 ortholog) alleles were tested for their defects in mitotic and meiotic mismatch repair. Mitotic mutation rates were similarly increased in msh6 and msh2 mutants, both for reversion of a base-base substitution as well as of an insertion of one nucleotide in a mononucleotide run. Tetrad analysis and intragenic two-factor crosses revealed that meiotic mismatch repair was affected in msh6 to the same extent as in msh2 background. In contrast, loss of Swi4 likely did not cause a defect in mismatch repair, but rather resulted in reduced recombination frequency. Consistently, a mutated swi4 caused a two- to threefold reduction of recombinants in intergenic crosses, while msh2 and msh6 mutants were not significantly different from wild type. In summary, our study showed that Msh6 plays the same important role as Msh2 in the major mismatch repair pathway of S. pombe, while Swi4 rather functions in recombination. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/11333218; hal-02491014; https://hal.science/hal-02491014Test; PUBMED: 11333218 |
| الإتاحة: | https://hal.science/hal-02491014Test |
| رقم الانضمام: | edsbas.66E17C62 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |