Robust Enhancement of Lentivirus Production by Promoter Activation
| العنوان: | Robust Enhancement of Lentivirus Production by Promoter Activation |
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| المؤلفون: | Ryuta Sakuma, Sayaka Sukegawa, Naoto Suzuki, Takeshi Yoshida, Shoji Yamaoka, Hiroaki Takeuchi |
| المصدر: | Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018) Scientific Reports |
| بيانات النشر: | Nature Portfolio, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Really Interesting New Gene (RING), Science, Genetic Vectors, Suppressor of Cytokine Signaling Proteins, Expressing Firefly Luciferase, Biology, CREB, Transfection, Virus Replication, Article, Viral vector, Cell Line, 03 medical and health sciences, Transduction (genetics), Transduction, Genetic, Lentiviral Vector Production, SPRY Domain, Humans, Promoter Regions, Genetic, Transcription factor, Human T-lymphotropic virus 1, Multidisciplinary, Lentivirus, Gene Transfer Techniques, Terminal Repeat Sequences, Promoter, Gene Products, tax, Vesicular Stomatitis Virus Glycoprotein (VSV-G), Cell biology, 030104 developmental biology, Viral replication, Cell culture, Host-Pathogen Interactions, biology.protein, HIV-1, Lentivirus Infections, Medicine, Genetic Engineering, Protein Binding |
| الوصف: | Lentiviral vectors are a valuable tool to deliver exogenous genes for stable expression in cells. While much progress has been made in processing lentiviral vector-containing culture medium, it remains to be explored how the production of lentiviral vector from producer cells can be increased. We initially found that co-expression of the SPRY domain-containing SOCS box protein 1 (SPSB1) promotes the production of human immunodeficiency virus type 1 (HIV-1) and lentiviral vector with increased expression of the Gag and envelope proteins and activation of the HIV-1 LTR and CMV promoter. The presence of AP-1, NF-κB and CREB/ATF recognition sites in these promoters prompted us to utilize human T-lymphotropic virus type 1 (HTLV-1) Tax for lentiviral vector production because Tax activates all these transcription factors. Co-expression of a small amount of Tax markedly increased both the expression of viral structural proteins in producer cells and release of lentiviral vector particles, resulting in a more than 10-fold enhancement of transduction efficiency. Of note, the Tax protein was not detected in the lentiviral vector particles concentrated by ultracentrifugation, supporting the safety of this preparation. Collectively, these results indicate that promoter activation in producer cells represents a promising approach to preparing high-titer lentiviral vectors. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::153835f63e7e560e256029109fd2f8dcTest https://doaj.org/article/e880902b6ac14fb2b80677fa0462439cTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....153835f63e7e560e256029109fd2f8dc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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