دورية أكاديمية
Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2
| العنوان: | Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2 |
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| المؤلفون: | Berini, Carolina Andrea, Susana Pascuccio, M., Bautista, Christian T., Gendler, Silvina A., Eirin, Maria Emilia, Rodriguez, Claudia, Pando, María de los Ángeles, Biglione, Mirna Marcela |
| بيانات النشر: | Elsevier Science |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | ASSAYS, COMPARISON, HTLV-1/2, SENSITIVITY, SPECIFICITY, https://purl.org/becyt/ford/1.6Test, https://purl.org/becyt/ford/1Test |
| الوصف: | Serological assays for human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) are widely used in routine screening of blood donors. The aim of this study was to compare the performance of four commercial screening assays for HTLV-1/2 infection frequently used in South America. A total of 142 HTLV-1 and HTLV-2 seropositive and 336 seronegative samples were analyzed by using four commercial tests (BioKit, Vironostika, Murex and Fujirebio). These tests are commonly used for HTLV-1/2 detection in blood banks in Argentina. A nested-PCR was used as the reference standard. The most sensitive tests for HTLV-1/2 were Fujirebio and Biokit (98.6%) followed by Murex (97.2%) and Vironostika (96.5%). The most specific test was Murex (99.7%), followed by Biokit (97.0%), Fujirebio (95.8%), and Vironostika (92.9%). The kappa index of agreement was higher for Murex (κ = 0.97), followed by BioKit (κ = 0.94), Fujirebio (κ = 0.92), and Vironostika (κ = 0.86). The highest index of agreement was shown by Murex test while Vironostika had the lowest performance. Of the four tests evaluated, only the Vironostika assay is approved by the Food and Drug Administration. These results should be considered for choosing the most accurate serological screening assays in order to obtain an optimal efficiency of the current algorithm for HTLV-1/2 diagnosis. ; Fil: Berini, Carolina Andrea. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina ; Fil: Susana Pascuccio, M. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina ; Fil: Bautista, Christian T. Asociacion Benefica Prisma Lima; Perú ; Fil: Gendler, Silvina A. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina ; Fil: Eirin, Maria Emilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0166-0934 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0166093407003734Test; http://hdl.handle.net/11336/112833Test; Berini, Carolina Andrea; Susana Pascuccio, M.; Bautista, Christian T.; Gendler, Silvina A.; Eirin, Maria Emilia; et al.; Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2; Elsevier Science; Journal of Virological Methods; 147; 2; 2-2008; 322-327; CONICET Digital; CONICET |
| الإتاحة: | https://doi.org/10.1016/j.jviromet.2007.09.012Test http://hdl.handle.net/11336/112833Test |
| حقوق: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by-nc-sa/2.5/arTest/ |
| رقم الانضمام: | edsbas.26CA3E6F |
| قاعدة البيانات: | BASE |
| تدمد: | 01660934 |
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