التفاصيل البيبلوغرافية
| العنوان: |
Microelectrode Array-evaluation of Neurotoxic Effects of Magnesium as an Implantable Biomaterial. |
| المؤلفون: |
Huang, Ting, Wang, Zhonghai, Wei, Lina, Kindy, Mark, Zheng, Yufeng, Xi, Tingfei, Gao, Bruce Z. |
| المصدر: |
Journal of Materials Science & Technology; Jan2016, Vol. 32 Issue 1, p89-96, 8p |
| مصطلحات موضوعية: |
MAGNESIUM, TETRAZOLIUM, MICROELECTRODES, NEUROTOXICOLOGY, BIOMATERIALS, CORROSION & anti-corrosives, LACTATE dehydrogenase |
| مستخلص: |
Magnesium (Mg)-based biomaterials have shown great potential in clinical applications. However, the cytotoxic effects of excessive Mg 2+ and the corrosion products from Mg-based biomaterials, particularly their effects on neurons, have been little studied. Although viability tests are most commonly used, a functional evaluation is critically needed. Here, both methyl thiazolyl tetrazolium (MTT) and lactate dehydrogenase (LDH) assays were used to test the effect of Mg 2+ and Mg-extract solution on neuronal viability. Microelectrode arrays (MEAs), which provide long-term, real-time recording of extracellular electrophysiological signals of in vitro neuronal networks, were used to test for toxic effects. The minimum effective concentrations (EC min ) of Mg 2+ from the MTT and LDH assays were 3 mmol/L and 100 mmol/L, respectively, while the EC min obtained from the MEA assay was 0.1 mmol/L. MEA data revealed significant loss of neuronal network activity when the culture was exposed to 25% Mg-extract solution, a concentration that did not affect neuronal viability. For evaluating the biocompatibility of Mg-based biomaterials with neurons, MEA electrophysiological testing is a more precise method than basic cell-viability testing. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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