التفاصيل البيبلوغرافية
| العنوان: |
Ovlivnění funkce ischemicky poškozených orgánů použitím perfluorocarbonu (PFC) jako konzervačního roztoku při experimentální transplantaci pankreatu, ledviny a Langerhansových ostrůvků |
| العنوان البديل: |
Posttransplant function of ischemically impaired organs (pancreas, kidney, islets) preserved by perfluorocarbon (PFC) |
| المؤلفون: |
Marada, Tomáš |
| مرشدي الرسالة: |
Adamec, Miloš, Špatenka, Jaroslav, Kříž, Jan |
| سنة النشر: |
2013 |
| المجموعة: |
Czech ETDs |
| مصطلحات موضوعية: |
apoptóza - ischemicko-reperfuzní poškození - konzervace - Langerhansovy ostrůvky - ledvina - pankreas - perfluorocarbon - potkan - roztok University of Wisconsin - two-layer method, apoptosis - ischemical-reperfusion injury - islet of Langerhans - kidney - pancreas - perfluorocarbon - preservation - rat - two-layer method - University of Wisconsin solution |
| الوصف: |
(English) Perfluorocarbons (PFC) are hydrocarbons in which some or all of the hydrogen atoms are replaced with fluorine. PFC have a very high capacity for dissolving oxygen. They are chemically and biologically inert. The most successful clinical application of PFC is the "two-layer method" for pancreas preservation before islet isolation. The two-layer organ preservation method (TLM) is based on oxygenated perfluorocarbon overlaid with University of Wisconsin (UW) solution. In experiment it has been successfully used for heart and intestine transplantation. We tested whether this technique would prevent tissue damage and improve results of kidney, pancreas and islets of Langerhans transplantation with prolonged ischemia time in an experimental model of syngenic rats. In kidney and islets of Langerhanse transplantation model we used TLM preservation method. In pancreas transplantation model we used perfluorohexyloctane (PFH) as a new generation of less lipophilic PFC. 1. Kidneys were stored for 24 hours either in UW solution (n = 16), with TLM (n = 16) or transplanted immediately (control group, n = 12). In half of the animals, survival was observed and in the other animals grafts were procured for semiquantitative histological scoring and TUNEL apoptosis assessment 24 h after transplantation... |
| Original Identifier: |
oai:invenio.nusl.cz:322185 |
| نوع الوثيقة: |
doctoralThesis |
| اللغة: |
Czech |
| الإتاحة: |
http://www.nusl.cz/ntk/nusl-322185Test |
| حقوق: |
info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: |
edsndl.nusl.cz.oai.invenio.nusl.cz.322185 |
| قاعدة البيانات: |
Networked Digital Library of Theses & Dissertations |
