المؤلفون: Antonija Perović, Jerka Dumić, Tihomir Balog, Sanja Dabelić, Sandra Sobočanec

المصدر: Free Radical Research

مصطلحات موضوعية: 0301 basic medicine, Adult, Male, diving, hyperoxia, oxidative stress, sirtuin 1, sirtuin 3, Antioxidant, Erythrocytes, SIRT3, medicine.medical_treatment, Diving, Physiology, medicine.disease_cause, Biochemistry, Peripheral blood mononuclear cell, Antioxidants, Oxidative damage, 03 medical and health sciences, 0302 clinical medicine, Superoxide Dismutase-1, Sirtuin 1, Sirtuin 3, Medicine, Humans, business.industry, Superoxide Dismutase, Oxidant antioxidant, 030229 sport sciences, General Medicine, Middle Aged, Catalase, Oxidants, Scuba diving, INTERDISCIPLINARY AREAS OF KNOWLEDGE, Oxidative Stress, 030104 developmental biology, Gene Expression Regulation, BIOMEDICINE AND HEALTHCARE, Leukocytes, Mononuclear, Lipid Peroxidation, Seasons, business, human activities, Oxidative stress

الوصف: The aim of this study was to examine the effects of scuba diving on oxidative damage markers in erythrocytes and plasma, antioxidant system in peripheral blood mononuclear cells (PBMCs), as well as sirtuin 1 (SIRT1) and sirtuin 3 (SIRT3) gene expressions in recreational divers after a winter nondive period (at least 5 months). For that purpose, 17 male recreational divers performed an immersion at a depth of 30 m for 30 min. Blood samples were collected immediately before and after diving, 3 and 6 h after diving. Erythrocyte lipid peroxidation measured by thiobarbituric-reactive substances (TBARS) method was significantly increased immediately after diving, but returned to the baseline 6 h after diving, while no significant change was found for plasma TBARS and protein carbonyl derivates in both plasma and erythrocytes. Diving-induced catalase (CAT), superoxide dismutase 2 (SOD2), and consequently total superoxide dismutase (SOD) activities in the PBMC samples (significantly increased immediately after diving, reached the maximum activities 3 h after diving, while 6 h after diving only CAT activity remained significantly increased). No significant change was observed for SOD1 activity and gene expression, as well as SOD2 expression, while CAT and SIRT1 expressions were slightly decreased immediately after diving and 3 h after diving. Interestingly, SIRT3 expression was significantly increased 6 h after diving. In conclusion, after the first dive to 30 m after a nondive season, activation of antioxidant defence was not sufficient to prevent oxidative damage, while SIRT3 upregulation could be a step towards an adaptive response to the diving.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c3164050980fc4e67b0f97f5a47aa94Test
http://fulir.irb.hr/3927Test/

المؤلفون: Damir Marjanović, Adna Ašić, Ramona Salazar, Wolfgang Höppner, Niels Storm, Dragan Primorac, Serkan Dogan

المصدر: Croatian Medical Journal
Volume 60
Issue 3

مصطلحات موضوعية: Male, pharmacogenetic markers, Gastroenterology, Thrombophilic markers, 0302 clinical medicine, BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences. Human Genetics, Genomics and Proteomics, Gene Frequency, Pregnancy, Medicine, Thrombophilia, 030212 general & internal medicine, BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti. Interna medicina, education.field_of_study, biology, Factor XIII, General Medicine, Middle Aged, Blood Coagulation Factors, Female, Prothrombin, VKORC1, BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti. Genetika, genomika i proteomika čovjeka, medicine.drug, Adult, medicine.medical_specialty, Adolescent, Genotype, Population, 03 medical and health sciences, Young Adult, 11th ISABS CONFERENCE, Internal medicine, Vitamin K Epoxide Reductases, thrombophilic markers, warfarin, Plasminogen Activator Inhibitor 1, Factor V Leiden, Humans, education, Allele frequency, BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences. Internal Medicine, Alleles, Methylenetetrahydrofolate Reductase (NADPH2), Cytochrome P-450 CYP2C9, Bosnia and Herzegovina, business.industry, Warfarin, Anticoagulants, Factor V, medicine.disease, Methylenetetrahydrofolate reductase, biology.protein, business, Pharmacogenetics, Biomarkers

الوصف: Aim To investigate the prevalence of common genetic variants that can serve as markers of thrombophilia and warfarin pharmacogenetics in Bosnia and Herzegovina. Methods The study was performed between August and October 2017 on 130 healthy unrelated adult volunteers from Bosnian-Herzegovinian population sample. The prevalence of the following genetic variants was determined: F5 c.1601G>A (factor V Leiden), F2 c.*97G>A (factor II or prothrombin mutation), F13A1 (factor XIII) c.103G>T, MTHFR (methylenetetrahydrofolate reductase) c.665C>T and c.1286A>C, as well as PAI-1 (plasminogen activator inhibitor 1) c.-816A>G and c.-844G>A as markers of thrombophilia risk, and *2 and *3 alleles of CYP2C9 (cytochrome P450 2C9) and five variants of VKORC1 (vitamin K epoxide reductase complex subunit 1) as markers of warfarin pharmacogenetics. DNA was isolated from buccal swabs using salting out method, while genotyping was performed using matrix-assisted laser desorption/ionization-time-offlight mass spectrometry. Results Minor allele frequencies for two main thrombophilia risk factors, F5 c.1601G>A and F2 c.*97G>A were 0.023 and 0.008, respectively. Combined data for the markers of warfarin pharmacogenetics imply that 57.4% study participants can be expected to metabolize warfarin at an extensive, 40.3% at intermediate, and 2.3% at a poor rate. Conclusion This study reports the first extensive population genetic data for thrombophilia and warfarin pharmacogenetic markers in Bosnia and Herzegovina. Allele frequencies of genetic variants are within the general average for European populations, and their presence implies the necessity of introduction of personalized medicine in warfarin-mediated antithrombotic therapy.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7b18db477761808c54dcf1611ee72b0Test
https://urn.nsk.hr/urn:nbn:hr:184:188436Test