دورية أكاديمية
Change in amount of serum cell-free mitochondrial DNA and clinical relevance in trauma patients
| العنوان: | Change in amount of serum cell-free mitochondrial DNA and clinical relevance in trauma patients |
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| المؤلفون: | GUO Enwei, REN Dali, ZHANG Bingyu, YANG feng, YAO Yulan, JIA Ling, YU Lin, FENG Gang |
| المصدر: | Waike lilun yu shijian, Vol 25, Iss 04, Pp 315-321 (2020) |
| بيانات النشر: | Editorial Office of Journal of Surgery Concepts & Practice, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens LCC:Surgery |
| مصطلحات موضوعية: | trauma, cell-free mitochondrial dna, c-reactive protein, systemic inflammatory response syndrome, cytokine, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Surgery, RD1-811 |
| الوصف: | Objective To investigate change in amount of serum cell-free mitochondrial DNA (cf-mtDNA) related with inflammatory response and to explore clinical relevance to trauma patients. Methods Thirty-seven trauma patients as trauma group were divided as multiple trauma subgroup or single trauma subgroup, and as shock subgroup or non-shock subgroup. Ten healthy volunteers were as control group. The amount of cf-mtDNA in serum at 4 h, 24 h, 72 h, 7 d post-injury was compared between trauma group and control group and among subgroups. The association of serum cf-mtDNA with injury severity score (ISS), systemic inflammatory response syndrome (SIRS) score, C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were analyzed respectively. The receiver operating characteristic (ROC) curve of serum cf-mtDNA post-injury were constructed to evaluate role in diagnosing SIRS. Results Serum cf-mtDNA in trauma patients at 24 h, 72 h post-injury were significantly higher than those in control group (P0.05). Amounts of TNF-α, IL-6 and CRP in serum increased with serum cf-mtDNA post-injury. Amount of serum cf-mtDNA correlated positively with ISS at 24 h post-injury (r=0.454, P=0.004), with SIRS score and serum IL-6 at 4 h-7 d post-injury respectively(r=0.458, P=0.000 1; r=0.252, P=0.005), but did not correlate with serum TNF-α (r=-0.058, P=0.511), and with CRP at 4-24 h post-injury positively (r=0.264, P=0.028). The area under ROC curve of serum cf-mtDNA was 0.752 at 4 h-7 d post-injury (P=0.000 01), with 95% confidence interval of 0.668-0.836. According to maximum Youden index, the optimal cutoff value of serum cf-mtDNA was 0.075 2 with the sensitivity 63.6% and specificity 85.5% for diagnosis of SIRS. Conclusions The amount of serum cf-mtDNA increased in trauma patients at early stage, indicating severe trauma and inflammation, and more amount of serum cf-mtDNA would be helpful to diagnose SIRS. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Chinese |
| تدمد: | 1007-9610 |
| العلاقة: | https://www.qk.sjtu.edu.cn/jscp/fileup/1007-9610/PDF/1658114166618-125295969.pdfTest; https://doaj.org/toc/1007-9610Test |
| DOI: | 10.16139/j.1007-9610.2020.04.010 |
| الوصول الحر: | https://doaj.org/article/af77ca51c6ac4aa988bd59615d553f61Test |
| رقم الانضمام: | edsdoj.f77ca51c6ac4aa988bd59615d553f61 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 10079610 |
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| DOI: | 10.16139/j.1007-9610.2020.04.010 |