| الوصف: |
Uric acid is metabolized from purine by the xanthine oxidase (XO) and excreted by urine. High level of serum uric acid leads to hyperuricemia and its complications such as gout, hypertension and chronic renal disease. Hsian-tsao (Mesona) is an annual herbaceous plant belonging to the Labiatae family, which has been reported to possess many beneficial properties, such as, anti-hypertension, hypoglycemic effect, hepatoprotective effect and improvement of diabetic myocardium impairment. However, the inhibitory effects of Hsian-tsao on hyperuricemia and kidney inflammation remain unclear. Therefore, we investigated the potential effect of Hsian-tsao on hyperuricemia and kidney inflammation in vitro and in vivo. We analyzed the active compounds and investigated the effects of Taiwan, Vietnam and China Hsian-tsao extracts after being extracted by water and various concentrations of ethanol (25, 50, 75 and 95%), respectively, on uric acid production and kidney inflammation, the results showed that China Hsian-tsao extracts contained the highest levels of phenolics and flavonoids. The results from HPLC analysis showed that p-hydroxybenzoic acid, caffeic acid and protocatechuic acid (PCA) were detected in Taiwan Hsian-tsao extracts, and the caffeic acid, PCA and hesperidin were detected in Vietnam Hsian-tsao extracts. However, China Hsian-tsao extracts contained caffeic acid only. In addition, both oleanolic acid and ursolic acid, the triterpenoids, presented in Hsian-tsao extracts from Taiwan, Vietnam and China. The in vitro result showed that Taiwan Hsian-tsao extracts exerted the greatest inhibitory effects on uric acid production. We further evaluated the inhibition of Taiwan Hsian-tsao extract on XO activity in THP-1 monocytes. The results showed that Taiwan Hsian-tsao extracts significantly inhibited the XO activity in monosodium urate (MSU)-induced THP-1 monocytes, and the 50% ethanol extracts (EE50) of Taiwan Hsian-tsao exhibited the most potent inhibitory activity. Because chronic kidney inflammation is one of ... |
