التفاصيل البيبلوغرافية
| العنوان: |
稻田金背鲤尾柄肌纤维特征及相关代谢酶与基因表达研究. (Chinese) |
| العنوان البديل: |
Study on muscle fiber characteristics, metabolic enzymes and gene expression of caudal stalk of golden-backed carp (Cyprinus carpio var. Jinbei). (English) |
| المؤلفون: |
张文争, 杨 立, 姚俊杰, 蒙庆米, 纪 达 |
| المصدر: |
South China Fisheries Science; Aug2023, Vol. 19 Issue 4, p77-85, 9p |
| مصطلحات موضوعية: |
PADDY fields, MOLECULAR biology, GENE expression, CARP, LACTATE dehydrogenase, NATURAL products |
| الملخص (بالإنجليزية): |
Golden-backed carp (Cyprinus carpio var. Jinbei) is a common carp that has lived in rice fields for many generations through long-term natural and artificial selection. In order to promote its paddy field breeding model and provide data supports for the breeding of improved varieties by this model, we analyzed the caudal stalk muscle fibers, related metabolic enzymes and gene expressions by using histology, enzymology and molecular biology methods, and explored the adaptive characteristics of caudal stalk muscle growth under the shallow ecological conditions of rice field. The results show that the diameter of caudal stalk muscle fiber in paddy field group was larger than that in pond group at flowering stage and harvest stage with significant differences (P<0.05). The cross-section area of caudal stalk muscle fiber in paddy field group was larger than that in pond group at flowering stage and harvest stage, with significant differences at harvest stage (P<0.05), while the density of caudal stalk muscle fiber in paddy field group was smaller than that in pond group. There were significant differences at harvest stage (P<0.05). The activities of lactate dehydrogenase (LDH), catalase (CAT) and Ca2+-ATPase in metabolic enzymes were higher in paddy field group than in pond group at flowering stage and harvest stage. The expression level of peroxisome proliferation-activated receptor γ-coactivator α (PGC-1α) gene in paddy field group was significantly higher than that in pond group (P<0.05). The results reveal that the expression levels of adenylate activated protein kinase (AMPK) and silence-regulating factor 1 (SIRT1) gene in paddy field group were significantly lower than those in pond group at flowering stage and harvest stage (P<0.05). The resistance movement of the caudal stalk of golden-backed carp in rice field caused muscle hypertrophy, which was related with the activation of protein synthesis pathway, while its endurance exercise, was related with the mitochondrial biosynthesis. The higher PGC-1α expression level of golden-backed carp in rice flowering stage may be related with the increase of rice flowering, insects, biodiversity and biomass. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
稻田金背鲤 (Cyprinus carpio var. Jinbei) 是经过多个世代自然选择和人工选择生活于稻田的鲤鱼。为推广金背鲤稻田养殖模式，为该模式下的良种选育提供数据支撑，以池塘养殖的金背鲤为对照组，采用组织学、酶学及分子生物 学方法分析了其尾柄肌纤维及相关代谢酶与基因表达，探究了稻田金背鲤尾柄肌肉在稻田浅水生态条件下生长的适应 性特征。结果显示：在水稻扬花期和渔获期，尾柄肌纤维直径稻田组均大于池塘组，并存在显著性差异 (P<0.05)；在 水稻扬花期和渔获期，尾柄肌纤维横截面积稻田组大于池塘组，在渔获期存在显著性差异 (P<0.05)；尾柄肌纤维密度 稻田组均小于池塘组，在渔获期存在显著性差异 (P<0.05)；在水稻扬花期和渔获期，代谢酶中的乳酸脱氢酶 (LDH)、 过氧化氢酶 (CAT) 和Ca2+-ATP酶活性稻田组均高于池塘组；在水稻扬花期，稻田组的过氧化物酶体增殖物激活受体γ辅 助激活因子α (PGC-1α) 基因表达量显著高于池塘组 (P<0.05)，而稻田组的腺苷酸活化蛋白激酶 (AMPK)、沉默信息调节 因子1 (SIRT1) 基因表达量在水稻扬花期和渔获期均显著低于池塘组 (P<0.05)。研究显示，稻田金背鲤尾柄肌肉有更大 的肌纤维直径和横截面积，尾柄肌肉呈现较高的LDH和Ca2+-ATP酶活性，这是其适应稻田浅水生活的特点；稻田金背 鲤的尾柄抗阻运动会引起肌肉肥大，与其激活蛋白质合成通路有关，而池塘金背鲤通常进行长时间的耐力运动，与其 线粒体生物合成有关；在水稻扬花期，稻田金背鲤PGC-1α基因表达量较高，可能与此阶段水稻开花、稻田中昆虫增 多、生物多样性增加以及生物量增加有关。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
