التفاصيل البيبلوغرافية
| العنوان: |
川芎嗪对脂多糖诱导大鼠心肌细胞炎症及氧化应激的作用. (Chinese) |
| العنوان البديل: |
Effects of ligustrazine on inflammation and oxidative stress in rat cardiomyocytes induced by lipopolysaccharide. (English) |
| المؤلفون: |
孙芳园, 孟佳磊, 马宇慧, 耿 欢, 张 涛 |
| المصدر: |
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu; 7/18/2023, Vol. 27 Issue 20, p3253-3258, 6p |
| مصطلحات موضوعية: |
TUMOR necrosis factors, REACTIVE oxygen species, LACTATE dehydrogenase, PROPIDIUM iodide, INTERLEUKIN-1 receptors, OXIDATIVE stress, MYELOID differentiation factor 88 |
| الملخص (بالإنجليزية): |
BACKGROUND: Sepsis-induced myocardial injury is related to inflammatory response and oxidative stress injury. Previous studies have shown that ligustrazine has many functions such as anti-oxidation and maintaining calcium homeostasis. OBJECTIVE: To investigate the effects of ligustrazine on inflammation and oxidative stress in lipopolysaccharide-induced rat cardiomyocytes. METHODS: Rat cardiomyocytes were induced by lipopolysaccharide (1 μg/mL) to establish septic cardiomyocyte inflammatory models. Twenty-four hours after intervention with different concentrations of ligustrazine (40, 80, 160 μmol/L), the samples were collected and detected. The effects of ligustrazine on the release of lactate dehydrogenase and reactive oxygen species from H9C2 cells induced by lipopolysaccharide were detected. Apoptosis was observed by fluorescence microscope. RT-qPCR was used to detect the changes of inflammatory factors such as interleukin-1 β, tumor necrosis factor-α, and interleukin-6 as well as the expression of Toll like receptor 4 and MyD88. RESULTS AND CONCLUSION: Compared with the normal control group, the expression of lactate dehydrogenase and reactive oxygen species in H9C2 cells increased significantly in the model group (P < 0.05) and a large number of cells were stained with propidium iodide under fluorescence microscope. The expression levels of interleukin-1β, tumor necrosis factor-α, and interleukin-6 were significantly increased (P < 0.05) and the expression of Toll like receptor 4 and MyD88 genes were significantly increased in the model group compared with the normal control group (P < 0.05). Compared with the model group, ligustrazine treatment could effectively inhibit the release of lactate dehydrogenase and reactive oxygen species, reduce the number of H9C2 cells stained by propidium iodide, downregulate the expression of interleukin-1β, tumor necrosis factor α and interleukin-6, and suppress the expression of Toll like receptor 4 and MyD88 genes (P < 0.05). To conclude, ligustrazine may reduce intracellular inflammatory factors and oxidative stress by inhibiting the expression of Toll like receptor 4/MyD88. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
背景：脓毒症心肌损伤与炎症反应及氧化应激损伤有关。既往研究表明川芎嗪具有抗氧化、维持钙稳态等多种功能。 目的：探讨川芎嗪对脂多糖诱导的大鼠心肌细胞炎症反应及氧化应激作用的影响。 方法：利用脂多糖(1 μg/mL)诱导大鼠心肌细胞建立脓毒症心肌细胞炎性模型，通过不同浓度的川芎嗪(40，80，160 μmol/L)干预治疗，24 h 后进行检测。利用试剂盒检测川芎嗪对脂多糖诱导H9C2细胞乳酸脱氢酶以及活性氧释放的影响，荧光显微镜观察细胞凋亡情况，检测炎 症因子白细胞介素1β、肿瘤坏死因子α和白细胞介素6及Toll样受体4、MyD88的表达变化。 结果与结论： 与对照组比较，模型组细胞中乳酸脱氢酶、活性氧表达水平显著上升(P < 0.05)，荧光显微镜下观察发现大量细胞被PI染 色，且白细胞介素1β、肿瘤坏死因子α和白细胞介素6的表达水平显著升高(P < 0.05)；Toll样受体4、MyD88基因表达均显著上升(P < 0.05)； 与模型组比较，川芎嗪治疗组能够有效降低乳酸脱氢酶释放，降低活性氧水平，抑制PI染色H9C2细胞数目，降低白细胞介素1β、肿瘤 坏死因子α和白细胞介素6的表达水平，抑制Toll样受体4、MyD88 mRNA及蛋白的表达(P < 0.05)； 提示川芎嗪可能通过抑制Toll样受体4/ MyD88的表达降低细胞的炎症因子及氧化应激反应。 [ABSTRACT FROM AUTHOR] |
|
Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
