التفاصيل البيبلوغرافية
| العنوان: |
脑心清对脑缺血再灌注损伤模型大鼠的保护机制. (Chinese) |
| العنوان البديل: |
Protective mechanism of Naoxinqing Capsule in rat models of cerebral ischemia/reperfusion injury. (English) |
| المؤلفون: |
闵冬雨, 李红岩, 关 乐, 常 江, 张海宁, 崔馨月, 王 鹏, 曹永刚 |
| المصدر: |
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu; 1/18/2020, Vol. 24 Issue 2, p215-222, 8p |
| مصطلحات موضوعية: |
NITRIC-oxide synthases, MONGOLIAN gerbil, CEREBRAL ischemia, REPERFUSION injury, LACTATE dehydrogenase, HIPPOCAMPUS (Brain), HINDLIMB, INTERLEUKIN-1 |
| الملخص (بالإنجليزية): |
BACKGROUND: Naoxinqing capsule has been used for treating cerebral ischemia/reperfusion injury for a long time. However, there are relatively few in-depth studies on its mechanism. OBJECTIVE: To investigate the therapeutic effect of Naoxinqing Capsule on gerbil model of cerebral ischemia/reperfusion injury by molecular biological means. METHODS: The study was approved by the Laboratory Animal Ethical Committee of Liaoning University of Chinese Medicine, approval No. 21000092017072. Eighty male Mongolian gerbils were randomly divided into sham, model, Naoxinqing and Naoluotong groups, and the latter three groups underwent bilateral common carotid artery clip for 5 minutes, to establish the model of cerebral ischemia/reperfusion injury. The sham group received no common carotid artery clip. Next day, the sham group fed normally, the model group was given normal saline, Naoxinqing group was given the 100 mg/(kg•d) Naoxinqing via gavage, and Naoluotong group given 100 mg/(kg•d) Naoluotong via gavage, respectively, for 21 consecutive days. The water maze test was conducted at 1 week before experiment ended. The brain tissue was removed after experiment. The learning and memory function, hippocampal neurons, cerebrovascular and corresponding molecular changes were detected. RESULTS AND CONCLUSION: (1) Compared with the sham group, the learning ability in the model group was decreased significantly. Naoxinqing and Naoluotong groups could effectively improve the learning ability after surgery. (2) Compared with the model group, the numbers of neurons in the Naoxinqing and Naoluotong groups were increased significantly, arranged regularly with clear contour and complete structure. (3) Compared with the model group, in the Naoxinqing and Naoluotong groups, the activities of superoxide dismutase and lactate dehydrogenase, and glutathione content were significantly increased, and the content of malonaldehyde was significantly decreased (P < 0.01). (4) The expression levels of ASC, NLRP3 and Caspase-1 in the hippocampus in the Naoxinqing and Naoluotong groups were significantly lower than those in the model group (P < 0.05). (5) The levels of interleukin-18 and interleukin-1β in the Naoxinqing and Naoluotong groups were significantly lower than those in the model group (P < 0.01). (6) Compared with the model group, the cells positive for platelet endothelial cell adhesion molecule-1 in the Naoxinqing and Naoluotong groups were significantly increased, the cells contacted closely each other. (7) Compared with the model group, in the Naoxinqing and Naoluotong groups, the expression levels of platelet endothelial cell adhesion molecule-1 and phosphorylated endothelial nitric oxide synthase were significantly up-regulated, and the content of nitric oxide was significantly increased (P < 0.01). (8) These results indicate that Naoxinqing and Naoluotong can effectively protect the morphology of hippocampal CA1 region in gerbils. Cerebral ischemia/reperfusion injury is accompanied by cerebral vascular dysfunction. Naoxinqing Capsule can protect cerebral vascular function and inhibit cerebral ischemia/reperfusion injury. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
背景：脑心清胶囊用于脑缺血再灌注损伤的治疗由来已久，然而针对其作用机制的深入研究则相对较少。 目的：应用分子生物学手段考察脑心清胶囊对脑缺血再灌注损伤沙鼠模型的治疗作用。 方法：实验方案经辽宁中医药大学动物实验伦理委员会批准(批准号为21000092017072)。将 80 只雄性蒙古 沙鼠随机分为假手术组、模型组、脑心清组及脑络通组，后 3 组沙鼠应用无创微动脉夹同时夹闭双侧颈总动 脉5 min 后松开，建立脑缺血再灌注损伤模型；假手术组不夹闭双侧颈总动脉。术后次日开始假手术组正常 饲养，模型组灌服同体积的生理盐水，脑心清组按照100 mg/(kg•d)灌胃给药，脑络通组按照100 mg/(kg•d) 灌胃给药，连续给药 21 d。在实验结束前1 周进行水迷宫实验，实验结束后麻醉下处死沙鼠取脑组织。检测 沙鼠的学习记忆功能、海马神经元、脑血管及对应的分子变化情况。 结果与结论：①同假手术组相比，模型组沙鼠学习能力显著下降。而脑心清组及脑络通组则可有效提升术后 的学习能力下降趋势；②与模型组相比，脑心清组及脑络通组神经元显著增多，且排列较为整齐，细胞轮廓 清晰，结构完整；③与模型组相比，脑心清组及脑络通组沙鼠的超氧化物歧化酶和乳酸脱氢酶活性，谷胱甘 肽含量显著升高，丙二醛含量显著降低(P < 0.01)；④与模型组相比，脑心清组及脑络通组沙鼠海马组织ASC、 NLRP3 和 Caspase-1 蛋白表达下调(P < 0.05)；⑤与模型组相比，脑心清组及脑络通组沙鼠的白细胞介素18 和白细胞介素1β 含量明显降低(P < 0.01)；⑥与模型组相比，脑心清组及脑络通组沙鼠的血小板内皮细胞黏 附分子1 阳性细胞明显增多，细胞间连接紧密；⑦与模型组相比，脑心清组及脑络通组沙鼠海马组织血小板 内皮细胞黏附分子1 和磷酸化内皮型一氧化氮合酶表达显著上调，一氧化氮含量显著升高(P < 0.01)；⑧结果 说明，脑心清胶囊可有效保护沙鼠的海马CA1 区形态；脑缺血再灌注时伴有脑血管功能紊乱，脑心清胶囊可 以保护脑血管功能，进而抑制脑缺血再灌注损伤。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
