دورية أكاديمية
缺血后适应对在体大鼠心肌缺血再灌注损伤的保护作用及其机制 ; Protective effect and mechanism of ischemic postconditioning in rats underwent myocardial ischemia/reperfusion injury
| العنوان: | 缺血后适应对在体大鼠心肌缺血再灌注损伤的保护作用及其机制 ; Protective effect and mechanism of ischemic postconditioning in rats underwent myocardial ischemia/reperfusion injury |
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| المؤلفون: | 张苗苗, 毛雯, 仝其广, 于海奕, 高炜 |
| المساهمون: | 煤炭总医院心内科, 北京,100028, 北京大学第三医院心内科 |
| المصدر: | CSCD ; 万方 ; http://d.g.wanfangdata.com.cn/Periodical_zhxxgb201607012.aspxTest |
| بيانات النشر: | 中华心血管病杂志 |
| سنة النشر: | 2016 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | 心肌再灌注损伤, 缺血后适应, Myocardial reperfusion injury, Ischemic postconditioning |
| الوصف: | 目的 探讨缺血后适应对在体大鼠缺血再灌注损伤后心肌梗死面积和血液动力学指标的影响及其机制.方法 将30只雄性SD大鼠按照随机数字表法分为假手术组、缺血再灌注组(IR组)和缺血后适应组(PC组),每组10只.通过结扎左冠状动脉前降支近段30 min,再灌注2h建立大鼠缺血再灌注模型(IR组).PC组为在再灌注开始瞬间实施再灌注30 s、缺血30 s,共3个循环,随后恢复再灌2h.通过颈动脉插管测定不同组大鼠的血液动力学指标;采用伊文思蓝和氯化三苯基四氮唑染色法测定缺血面积和梗死面积;分别通过Western blot和实时定量PCR检测各组大鼠再灌注2h缺血心肌预测的靶基因Bcl-2家族促凋亡蛋白之一Bim蛋白及微小RNA (miR)-214的表达水平.结果 (1)各组大鼠血液动力学的检测结果:IR组和PC组大鼠左心室收缩压(LVSP)、左心室内压最大上升速率(+dp/dtmax)及最大下降速率(-dp/dtmax)和心率均较假手术组低,左心室舒张末压(LVEDP)较假手术组高,差异均有统计学意义(P均＜0.05).PC组大鼠LVSP、+dp/dtmax和-dp/dtmax均较IR组高,LVEDP较IR组低,差异均有统计学意义(P均＜0.05).(2)各组大鼠心肌缺血和梗死范围的检测结果:PC组大鼠心肌缺血区面积(AAR)占左心室面积(LV)的比例(AAR/LV)与IP组比较差异无统计学意义[(27.00±7.55)％比(26.67±11.68)％,P＞0.05],梗死面积(IS)占AAR的比例(IS/AAR)则低于IR组[(30.67±3.51)％比(48.67±4.62)％,P＜0.05].(3)各组大鼠缺血心肌Bim蛋白表达的检测结果:IR组大鼠缺血区心肌组织中Bim蛋白表达水平高于假手术组(2.34 ±0.15比0.75 ±0.05,P＜0.05).PC组大鼠缺血区心肌组织中Bim蛋白表达水平则低于IR组(1.25 ±0.14比2.34 ±0.15,P＜0.05).(4)各组大鼠缺血心肌miR-214表达的检测结果:IR组大鼠缺血心肌组织中miR-214表达水平低于假手术组(0.20±0.04比1.00,P＜0.01),PC组大鼠缺血心肌组织中miR-214表达水平则高于IR组(0.85±0.20比0.20±0.04,P＜0.01),PC组与假手术组比较差异无统计学意义(P＞0.05).结论 缺血后适应处理可减少在体大鼠心肌梗死面积,改善缺血再灌注损伤后血液动力学,改善大鼠心肌缺血再灌注损伤.缺血后适应可降低Bim蛋白表达,Bim可能参与了缺血后适应的心肌保护作用,而其下调机制可能与miR-214表达升高有关. ; Objective This study is designed to observe the effect of ischemic postconditioning in rats underwent acute myocardial ischemia/reperfusion injury and to investigate the related mechanism.Methods A total of 30 SD rats were randomly divided into sham operation group (control group,n =10),ischemia/reperfusion group (IR group,n =10) and ischemic postconditioning group (PC group,n =10) based on random number table.Rats in IR group underwent 30 minutes myocardial ischemia by occlusion of the proximal portion of left anterior descending (LAD) coronary artery followed by 2 hours reperfusion.In control group,there was no IR intervention.In PC group,at the start of reperfusion,three cycles of 30 seconds reperfusion and 30 seconds LAD reocclusion preceded the 2 hours of reperfusion.The hemodynamic values were measured via a cannula inserted into the right common carotid artery.The area at risk was assessed by ... |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | Chinese |
| تدمد: | 0253-3758 |
| العلاقة: | 中华心血管病杂志.2016,44(7),616-620.; 1447259; http://hdl.handle.net/20.500.11897/494356Test |
| DOI: | 10.3760/cma.j.issn.0253-3758.2016.07.012 |
| الإتاحة: | https://doi.org/20.500.11897/494356Test https://doi.org/10.3760/cma.j.issn.0253-3758.2016.07.012Test https://hdl.handle.net/20.500.11897/494356Test |
| رقم الانضمام: | edsbas.F8515AE4 |
| قاعدة البيانات: | BASE |
| تدمد: | 02533758 |
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| DOI: | 10.3760/cma.j.issn.0253-3758.2016.07.012 |