تقرير
新穎纖維分解酶之開發與應用 ; Development and Application of the Novel Cellulases
| العنوان: | 新穎纖維分解酶之開發與應用 ; Development and Application of the Novel Cellulases |
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| المؤلفون: | 張敏政 |
| المساهمون: | 醫學系生物化學暨分子生物學研究所 |
| سنة النشر: | 2009 |
| المجموعة: | National Cheng Kung University: NCKU Institutional Repository / 國立成功大學機構典藏 |
| مصطلحات موضوعية: | 外泌蛋白, 巨基因, 蛋白質大量表現, 纖維素分解酶, Secreted Protein, Metagenome, Protein Overexpression, Cellulase |
| الوصف: | 計畫編號:98農科-1.2.1-科-ai ; 執行機構:成功大學醫學系生物化學暨分子生物學研究所 ; 研究期間:2009-03~2009-12 ; 纖維素是植物細胞壁的主要成分，也是蘊藏量最豐富的再生能源原料，而利用纖維素分解?可達到轉化為再生能源的目的亦不會造成環境的汙染。此外，纖維素分 解?亦可廣泛應用於食品、動物飼料、紡織及造紙等工業以及清潔劑市場上。由於目前已發現的纖維素分解?而未能應付廣泛市場所需，新穎纖維素分解?及其基因 能不斷被報告及研究。 在本團隊的初步成果中，我們由未經培養的巨基因中選殖出含有Ucel6A基因在內之四種纖維素分解?基因，除外，由於環境中不能培養的微生物仍佔絕大部 分，理論上蘊藏龐大的生化活性物質而未被發掘，因此本計劃之目標為大量表現之選殖的新穎纖維素分解?Ucel6A，決定其酵素特性以及篩選更多的新穎纖維 素分解?基因而開發其被應用於工業上多達此目的。本計劃之第一年擬進行以下之實驗項目即：(1)探討Ucel6A在大腸菌分泌機制(2)建構Ucel6A 在枯草菌之表現系統(3)Ucel6A酵素特性分析及蛋白質工程改良(4)大腸菌之菌體外量產Ucel6A之提升(5)特殊且新穎脂纖維素分解?基因選殖 我們期待成果為可選殖出更多且特殊而新穎的纖維素分解?基因，同時被大腸菌大量表現且分泌於菌體外以及被枯草菌大量表現的Ucel6A可達到應用於清潔劑 製造產業上的目標。 ; Cellulose is the most component of plant cell wall and is the most abundant renewable resource. Enzymatic hydrolysis of cellulose using cellulases is attractive because its conversion can be achieved without environment. In addition, cellulases are also used in the food, animal feed, textile and paper industries and in the detergent market. The search for new cellulases and genes continues, as those already discovered have not completely met the requirememts for the intented industrial application. In our previous study, four novel cellulase genes including ucel6A have been cloned from metagenomes of uncultured microorganisms. In addition, since there is a vast biocatalytic potential locked within the uncultured portion of envirmental microbial consortia, the goal of this proposal is to overexpress the recombinant Ucel6A and characterize its enzymatic property as well as to clone the novel cellulases for industrial uses. Thus, in the first year of this proposal, the following experiments will be performed.(1) Investigation of the secreted mechanism of Ucel6A in E. coli.(2) Establishment of Ucel6A-expressing in Bacillus subtilis(3) Characterization of Ucel6A’ enzymatic property and improving its enzymatic activities by using protein engineering(4) Enhancement of the extracellular Ucel6A production in E. coli.(5) Cloning of the metagenome-derived novel cellulase genesOur predicting achievement is that several novel ... |
| نوع الوثيقة: | report |
| اللغة: | Chinese English |
| العلاقة: | http://ir.lib.ncku.edu.tw/handle/987654321/104966Test |
| الإتاحة: | http://ir.lib.ncku.edu.tw/handle/987654321/104966Test |
| رقم الانضمام: | edsbas.2B9B8A4C |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |