Relationship between clinical features and intestinal microbiota in Chinese patients with ulcerative colitis
| العنوان: | Relationship between clinical features and intestinal microbiota in Chinese patients with ulcerative colitis |
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| المؤلفون: | Jingnan Li, Chu-Yan Chen, Peng-Guang Yan, Ke-Min Li, Xu-Xia He, Xiangchen Meng, Ying-He Li |
| المصدر: | World Journal of Gastroenterology |
| بيانات النشر: | Baishideng Publishing Group Inc, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | medicine.medical_specialty, Intermicrobial correlation, China, Intestinal microbiota, Observational Study, Disease, Gastroenterology, Feces, Internal medicine, RNA, Ribosomal, 16S, medicine, Humans, In patient, Intestinal Mucosa, Gastrointestinal tract, Chinese, Bacteria, business.industry, Lachnospiraceae, Significant difference, Fungi, General Medicine, medicine.disease, Ulcerative colitis, Gastrointestinal Microbiome, Dysbiosis, Colitis, Ulcerative, business, Ruminococcaceae |
| الوصف: | BACKGROUND Dysbacteriosis may be a crucial environmental factor for ulcerative colitis (UC). Further study is required on microbiota alterations in the gastrointestinal tract of patients with UC for better clinical management and treatment. AIM To analyze the relationship between different clinical features and the intestinal microbiota, including bacteria and fungi, in Chinese patients with UC. METHODS Eligible inpatients were enrolled from January 1, 2018 to June 30, 2019, and stool and mucosa samples were collected. UC was diagnosed by endoscopy, pathology, Mayo Score, and Montreal classification. Gene amplicon sequencing of 16S rRNA gene and fungal internal transcribed spacer gene was used to detect the intestinal microbiota composition. Alpha diversity, principal component analysis, similarity analysis, and Metastats analysis were employed to evaluate differences among groups. RESULTS A total of 89 patients with UC and 33 non-inflammatory bowel disease (IBD) controls were enrolled. For bacterial analysis, 72 stool and 48 mucosa samples were obtained from patients with UC and 21 stool and 12 mucosa samples were obtained from the controls. For fungal analysis, stool samples were obtained from 43 patients with UC and 15 controls. A significant difference existed between the fecal and mucosal bacteria of patients with UC. The α-diversity of intestinal bacteria and the relative abundance of some families, such as Lachnospiraceae and Ruminococcaceae, decreased with the increasing severity of bowel inflammation, while Escherichia-Shigella showed the opposite trend. More intermicrobial correlations in UC in remission than in active patients were observed. The bacteria-fungi correlations became single and uneven in patients with UC. CONCLUSION The intestinal bacteria flora of patients with UC differs significantly in terms of various sample types and disease activities. The intermicrobial correlations change in patients with UC compared with non-IBD controls. |
| اللغة: | English |
| تدمد: | 2219-2840 1007-9327 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf8f6e07e110d63d34d72b10806aa142Test http://europepmc.org/articles/PMC8326252Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bf8f6e07e110d63d34d72b10806aa142 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22192840 10079327 |
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